From 2007.igem.org

1.2% Agarose Gel preparation

• 8 wells
• 600mg Agarose
• 50mL TBE
• 1uL 10mg/ml EtBr

Mix agarose and TBE in flask, microwave for 20 second intervals and swirl it until solution is clear (usually 1-2min. total) to make sure agarose is dissolved in TBE. Add EtBr to flask (under hood) and pour into gel box with comb in place. Wait until gel solidifies.

• 2uL DNA
• 12uL ddH₂O
• 4uL 5x Loading Dye
• 4uL DNA Ladder for reference

Add water if DNA is less than 10uL and make sure #uL of 1x loading dye = #uL total. Mix content in tube before adding it to well. Also make sure gel box is submerged in TAE/TBE before adding DNA.