From 2007.igem.org

Discussion Page

This page is for questions, answers and discussions on the project, a kind of open forum.

• Test of DapA deletion transduction, Thursday, July 5

How did you test for the success of this transduction? Simply with a normal culture without supplemented lysine in the medium, and seeing that the bacteria grows neverthless? Or is it that in the original DapA- strain tha DapA gene was replaced by an antibiotic resistance gene, that should then be present in the transfected bacteria?

• For the modellers

Have a look at the Cambridge team wiki. They have a detailed page on [http://www.ccbi.cam.ac.uk/iGEM2007/index.php/Software_Modelling_for_Synthetic_Biology modelling and iGEM. ]

• For everybody

Have a look at the Edinburgh team wiki. They also present a Cre/Lox box in one of their constructions !

• Team Notebook and Protocols

I suggest that the Team Notebook section summarizes the work progress, the meaning of the different steps, and intermediate results. All protocols should be in the protocols section with links.

Question: how to link directly to a subsection of a page in a wiki?

Answer: click here to read July 2nd entry.

• Concerning the wiki

The notebook is now accessible via a calendar. I hope that you will prefer the notebook like that. I moved some pages from their places to Paris/, and it is a good idea to take care that every page we create begins by Paris/... Nicolas C.

Samuel B.: The calender is a nice feature, however isn't it possible to have also everything in a single page. It is useful to have an overall view of the notebook. There is a risk with wikis to split informations on too many pages. This is clearly the case by presenting all in a calender...

I discussed with Eimad and David (P) and the idea is to make two notebooks :

• one accessible via the calendar, where we put all that we do during the day (still separated from protocols), for the people in the lab
• another one which will summarize goals and progresses step by step. We will begin this one soon. Nicolas C.

• Have a look at the Boston team wiki main page

I find it very clear. Maybe a good inspiration for a next version of ours... Samuel B.

• Concerning kinetic measurements

I made a template in order to present growth experiments clearly when we use the 96 plate array. Click here to see an example.