Paris/July 12
From 2007.igem.org
< Paris
Revision as of 10:53, 12 July 2007 by David.bikard (Talk | contribs)
David B:
Preparation of a stock of phage on w121:
- Filtration of the Cacl2 and MgSO4 stocks
- Launch a culture:
- 15ml LB
- 225µl Cacl2
- 450µl MgSO4
- 90µl DAP
- 150µl W121 from an ON culture
- Let grow until OD=0.2 (~1h)
- Add 100µl P1 (stock 10^10/ml)
- Incubate 2h
- Add 1ml of the ON culture of W121
- Incubate 2h
- Add 200µl Chloroform
- Vortex, centrifuge (15min @ 3000rpm)
- Recover the supernatant = Stock keep at 4°C
