From 2007.igem.org

David B:

Preparation of a stock of phage on w121:

• Filtration of the Cacl2 and MgSO4 stocks
• Launch a culture:
  • 15ml LB
  • 225µl Cacl2
  • 450µl MgSO4
  • 90µl DAP
  • 150µl W121 from an ON culture
• Let grow until OD=0.2 (~1h)
• Add 100µl P1 (stock 10^10/ml)
• Incubate 2h
• Add 1ml of the ON culture of W121
• Incubate 2h
• Add 200µl Chloroform
• Vortex, centrifuge (15min @ 3000rpm)
• Recover the supernatant = Stock keep at 4°C

Nicolas C.

Titration of bacteriophages P1

We measure the strength of former P1 preparation :

• The stock prepared the 3.7.7
• The stock prepared the 8.7.7

See protocols for details.