Paris/What's going on in the lab
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Revision as of 15:49, 13 July 2007 by David.bikard (Talk | contribs)
See the Notebook
Contents |
What's new ?
We just received a first part of the oligos
To Do List
- Cloning of I0500 (inducible pBad) in [http://partsregistry.org/Part:BBa_J61002 J61002]
This will allow us to compare the strength of the pBad and the strengh of the constitutive promoter. Moreover, we'll be able to clone any gene we like after the promoter.
In Progress
FstZ strain caracterisation
We try to obtain a reliable TS clone, which would not grow at 42°C, but grow at 30°C
- Unsuccessful with the three clones we tested at the moment.
Transduction of the deletion of DapA from the strain w121 into MG1655 and MG1655 FtsZ TS84
The goal is, by transduction, to introduce DapA deletion into FtsZ TS strain
- We managed to build a stock of efficient phages (stock of 12.7.7)
- Transduction in progress
Transfection of 3 Biobricks
The goal is to extract DNA from the plates and make a stock of plasmid we need.
- BBa_I0500: Inducible pBad/araC in pSB2K3 (KanR)
- BBa_J23100: strong constitutive promoter in BBa_J61002 (AmpR)
- BBa_B0015: double terminator (B0010-B0012) in pSB1AK3 (AmpR)
