Paris/Modeling char

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Revision as of 13:37, 19 July 2007 by Davidoff (Talk | contribs)

We must characterise the different part we are using. Essentially :

  • The promoters:
  1. -DAPap: we must study the basic pops when no DAP is present, and establish the pops output curve depending of

repression. look the arabinose AraC

  1. -the high constitutive promoter that we are using:BBa_J23112
  • cre efficience :


BBa_J5528 :Arabinose -> pBad = GFP : use it to test pbad efficiency,or make a similar construct

assemble Arabinose and cre one one plasmide, loxP ccdb loxP on another, and test the recombination frequency


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