From 2007.igem.org

Home

Project Description

Methods

Notebook

Results and Discussion

Resources

Back to Mimi's Notebook

Sequences and Properties of New Oligonucleotides:

R-bchH: (Longer Oligonucleotide-to use resulting PCR fragment as template):

         R-bchH3-29bp
         5' - ATC ATC ACC CGG TTG AGC GTG CCC AGG AG - 3'
         TM (50mM NaCL): 68.4 °C
         GC Content: 62.1%

          R-bchH5-29bp
         5' - ATG TGC AGG ACT TCC CGG CCC GTT ACT CG - 3'
         TM (50mM NaCL): 68.2 °C
         GC Content: 62.1%

Yields a R-bchH fragment of 5877 base pairs

(Oligonucleotide used for resulting PCR fragment of longer oligo):

         R-bchH3-kpnI-bamHI-2
         5' - TAA AGG ATC CAA AGG TAC CTC ACT CGG CGG CAA TCC CCT CGA GAC GGT C - 3'
         TM (50mM NaCL): 71.5 °C
         GC Content: 57.1%
         
         R-bchH5-ndeI-3
         5' - AAT TCA TAT GCA CGG TGA AGT CTC AGG TCC AGC CGG C - 3'
         TM (50mM NaCL): 68.2 °C
         GC Content: 54.1%

Yields a R-bchH fragment of 3582 base pairs

R-bchI:

         R-bchI3-speI-NsiI-bglII-2
         5' - TAT TAG ATC TTT AAT GCA TTT TAC TAG TTC AGG GCA GGA CCT CCG CCA CGC ACC GTT C - 3'
         TM (50mM NaCL): 69.1 °C
         GC Content: 46.6%

         R-bchI5-kpnI-rbs-2
         5' - TTT AGG TAC CAA GAA GGA GAT ATA CTT ATG AAA AAA CCC TTC CCC TTC TCG GCC ATC - 3'
         TM (50mM NaCL): 67 °C
         GC Content: 42.1%

R-bchD:

         R-bchD3-bglII-2
         5' - TTA AAG ATC TTC AGT CTC CCA TTG CTG CGC CGA GGA CGT TC - 3'
         TM (50mM NaCL): 67.9 °C
         GC Content: 51.2%

         R-bchD5-SpeI-RBS-ATG
         5' - TAT AAC TAG TAA GAA GGA GAT ATA CTT ATG CCG CTC GGC CCG TGG GAA CGG GTC G - 3'
         TM (50mM NaCL): 69.4 °C
         GC Content: 50.9%