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Construction of pET3A Derivatives Containing R-bchH, R-bchI, and R-bchD (Simultaneous Ligation)

Rhodobacter sphaeroides

       R-bchH: 3582 base pairs
       R-bchI: 1005 base pairs
       R-bchD: 1695 base pairs

Sequences and Properties of Oligonucleotides for R-bch Genes

Construction of pET3A-(R)-bchHID - Not Successful

Sequences and Properties of Oligonucleotides for R-bch Genes(NEW)

Construction of pET3A-(R)-bchHID - 2nd Attempt - Not Successful

Contruction of pET3a Derivatives Containing S-chlH, S-chlI, and S-chlD (Sequential Ligation)

Cyanobacteria Synechocystis

       S-chlH: 3996 base pairs
       S-chlI: 1074 base pairs
       S-chlD: 2031 base pairs

Sequences and Properties of Oligonucleotides for S-chl Genes

Construction of pET3A-(S)-chlH

Construction of pET3A-(S)-chlHI

Construction of pET3A-(S)-chlHID