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Sequences and Properties of Oligonucleotides:

R-bchH:

         R-bchH3-KpnI-BamHI
         5' - TAA AGG ATC CAA AGG TAC CTC ACT CGG CGG CAA TCC CCT - 3'
         TM (50mM NaCL): 69.2 °C
         GC Content: 53.8%

         R-bchH5-ndeI
         5' - AAT TCA TAT GCA CGG TGA AGT CTC AGG - 3'
         TM (50mM NaCL): 58.8 °C
         GC Content: 44.4%

R-bchI:

         R-bchI3-SpeI-NsiI-BglII
         5' - TAT TAG ATC TTT AAT GCA TTT TAC TAG TTC AGG GCA GGA CCT CCG CCA CGC - 3'
         TM (50mM NaCL): 67.5 °C
         GC Content: 45.1%

         R-bchI5-KpnI-RBS
         5' - TTT AGG TAC CAA GAA GGA GAT ATA CTT ATG AAA AAA CCC TTC CCC TTC TCG GCC - 3'
         TM (50mM NaCL): 66.7 °C
         GC Content: 42.6%

R-bchD:

         R-bchD3-BamHI
         5' - TTA AGG ATC CTC AGT CTC CCA TTG CTG CGC C - 3'
         TM (50mM NaCL): 65.9 °C
         GC Content: 54.8%

         R-bchD5-SpeI-RBS-ATG
         5' - TAT AAC TAG TAA GAA GGA GAT ATA CTT ATG CCG CTC GGC CCG TGG GAA CGG GTC G - 3'
         TM (50mM NaCL): 69.4 °C
         GC Content: 50.9%