Melbourne/Lab BL Notebook/20071005PCR2
From 2007.igem.org
PCR reaction to attach prefix and suffix to the PsrfA promoter.
| PCR mix | PCR program |
|---|---|
| 5ul 10x buffer\\
2.5ul 10x Enhancer 0.6ul dNTPs (25mM stock) 2.5ul MgSO4 (Supplied in PCR kit) 1.5ul Primer BL_psrfA_fwd (10uM stock) 1.5ul Primer BL_psrfA_rev (10uM stock) 1ul Template (pJS34) 0.4ul Pfx Platinum (Invitrogen) 32.9ul ddH2O | 94°C - 5'
94°C - 30" 50°C - 35" 68°C - 1' (goto step 2 x30) 68°C -10' 4°C forever |
| 50ul Total |
=> No product. Need to play around with conditions.
