Paris/August 25
From 2007.igem.org
< Paris
GLYCEROL STOCKS
We made glycerol stocks of the W121 strains transformed with [DapAp>>mRFP] construction
| Strains glycerol stock | ||||
|---|---|---|---|---|
| Number | Name | Description | Verified clone | Date |
| S45A | L30A | DapAp>>mRFP within W121 strain | August 25 | |
| S45B | L30B | DapAp>>mRFP within W121 strain | August 25 | |
Recombination Rate Test
- We counted how many clones grew up on AMP-KAN :
- FX85 L26.1 : 78%
- FX85 L26.2 : 93%
In all cases, we are up to the 50% max necessary to permit the spread of the population. As François-Xavier told us, CRE seems really a too good worker for our stuff !
- ??? No clones from the dillution culture appeared on our AMP-THYMINE plates ??? We decide to try again with 10-7 and 10-5 dillution so as to check if the lack of clones would come from a too high dillution.
