BerkiGEM2007-AustinConstructionFile24

From 2007.igem.org

(Difference between revisions)
 
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Alpha: <br>
Alpha: <br>
PCR CA1150/CA1099R on 9145-I716077 (1727 Bp)<br>
PCR CA1150/CA1099R on 9145-I716077 (1727 Bp)<br>
-
Digest (Bsa1/Bgl2) (~2000/Small, Larger) and ligate with beta portion.<br> <br>
+
Digest (Bsa1/Bgl2) (2111/Small, Larger) and ligate with beta portion.<br> <br>
Beta: <br>
Beta: <br>

Latest revision as of 22:45, 10 July 2007

Alpha:
PCR CA1150/CA1099R on 9145-I716077 (1727 Bp)
Digest (Bsa1/Bgl2) (2111/Small, Larger) and ligate with beta portion.

Beta:

PCR CA1099F/G00101 on Part 9145-I716081
Digest (Bsa1/BamH1) (1447/small, Larger) and ligate with alpha portion.


Product is 9145-I716074 (best yield)