From 2007.igem.org

(final volume 20 μl)

1. Mix :

- 8 μl of insert DNA

- 4 μl of vector plasmid

- 4 μl of Buffer 5X [ ]

- 1 μl of T4 Ligase

- 3 μl of ultrapure water

2. Incubate at 4 °C overnigth or at 15 °C for 3 hr.

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