Bologna University/M9 medium
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< Bologna University(Difference between revisions)
| Line 1: | Line 1: | ||
'''Preparation instructions:''' | '''Preparation instructions:''' | ||
| - | ::1.Dissolve 56.4g in 1L of distilled water. | + | ::1. Dissolve 56.4g in 1L of distilled water. |
| - | ::2.Autoclave for 15min at 121°C. | + | ::2. Autoclave for 15min at 121°C. |
| - | This convenient 5x concentrate can be stored and | + | This convenient 5x concentrate can be stored and diluted as needed to prepare 5L of 1x M9 minimal salts. |
For M9 minimal medium: | For M9 minimal medium: | ||
| - | ::1.Aseptically dilute 200mL of M9 minimal salts, 5x | + | ::1. Aseptically dilute 200mL of M9 minimal salts, 5x [ ] with 800mL of sterile water, if necessary, cool to 45-50°C. |
| - | ::2.Aseptically add 20mL of sterile 1M glucose and 2mL of sterile 1M magnesium solfate to prepare 1L of M9 minimal medium. | + | ::2. Aseptically add 20mL of sterile 1M glucose and 2mL of sterile 1M magnesium solfate to prepare 1L of M9 minimal medium. |
| - | ::3.If desired aseptically add 0.1mL of 1M sterile calcium | + | ::3. If desired aseptically add 0.1mL of 1M sterile calcium chloride to the M9 minimal medium. M9 minimal medium may also be supplemented with the appropriate amino acids. |
[[Bologna University/Mediums and buffers | Back]] | [[Bologna University/Mediums and buffers | Back]] | ||
Latest revision as of 09:26, 25 October 2007
Preparation instructions:
- 1. Dissolve 56.4g in 1L of distilled water.
- 2. Autoclave for 15min at 121°C.
This convenient 5x concentrate can be stored and diluted as needed to prepare 5L of 1x M9 minimal salts. For M9 minimal medium:
- 1. Aseptically dilute 200mL of M9 minimal salts, 5x [ ] with 800mL of sterile water, if necessary, cool to 45-50°C.
- 2. Aseptically add 20mL of sterile 1M glucose and 2mL of sterile 1M magnesium solfate to prepare 1L of M9 minimal medium.
- 3. If desired aseptically add 0.1mL of 1M sterile calcium chloride to the M9 minimal medium. M9 minimal medium may also be supplemented with the appropriate amino acids.
