Freiburg07/primers
From 2007.igem.org
(Difference between revisions)
(→Primers for specific ends of the dhfr1-fragment) |
(→Primers for calmodulin-construct with different linkerlengths) |
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== Primers for calmodulin-construct with different linkerlengths == | == Primers for calmodulin-construct with different linkerlengths == | ||
| + | |||
| + | forward primers:<br> | ||
pf-calmo-KpnI-linker2: 5' TATCGACGGT ACCGGCGGTG AGCAGATTGC AGAGTTCAAA G 3' | pf-calmo-KpnI-linker2: 5' TATCGACGGT ACCGGCGGTG AGCAGATTGC AGAGTTCAAA G 3' | ||
| + | pf-calmo-KpnI-linker3: 5' TATCGACGGT ACCGGCGGTG GCGAGCAGAT TGCAGAGTTC AAAG 3' | ||
| + | pf-calmo-KpnI-linker4: 5' TATCGACGGT ACCGGCGGTG GCGGTGAGCA GATTGCAGAG TTCAAAG 3' | ||
| + | pf-calmo-KpnI-linker6: 5' TATCGACGGT ACCGGCGGTG GCTCTGGTGG CGAGCAGATT GCAGAGTTCA AAG 3' | ||
| + | pf-calmo-KpnI-linker9: 5' TATCGACGGT ACCGGCGGTG GCGGTTCTGG CGGTGGCGGT GAGCAGATTG CAGAGTTCAA AG 3' | ||
Revision as of 14:04, 22 October 2007
Primers for specific ends of the dhfr1-fragment
pr-Kpn1-dhfr1: 5' TAATTAGGTA CCCAATTCCG GTTGTTC 3'
pf-Sph-h-dhfr1: 5' TATCGACGCA TGCACCATCA CCATCACCAT ATGGTTCGAC CATTGAAC 3'
Primers for calmodulin-construct with different linkerlengths
forward primers:
pf-calmo-KpnI-linker2: 5' TATCGACGGT ACCGGCGGTG AGCAGATTGC AGAGTTCAAA G 3' pf-calmo-KpnI-linker3: 5' TATCGACGGT ACCGGCGGTG GCGAGCAGAT TGCAGAGTTC AAAG 3' pf-calmo-KpnI-linker4: 5' TATCGACGGT ACCGGCGGTG GCGGTGAGCA GATTGCAGAG TTCAAAG 3' pf-calmo-KpnI-linker6: 5' TATCGACGGT ACCGGCGGTG GCTCTGGTGG CGAGCAGATT GCAGAGTTCA AAG 3' pf-calmo-KpnI-linker9: 5' TATCGACGGT ACCGGCGGTG GCGGTTCTGG CGGTGGCGGT GAGCAGATTG CAGAGTTCAA AG 3'
