From 2007.igem.org

Primers for specific ends of the dhfr1-fragment

pr-Kpn1-dhfr1: 5' TAATTAGGTA CCCAATTCCG GTTGTTC 3'

pf-Sph-h-dhfr1: 5' TATCGACGCA TGCACCATCA CCATCACCAT ATGGTTCGAC CATTGAAC 3'

Primers for calmodulin-construct with different linkerlengths

forward primers:

pf-calmo-KpnI-linker2: 5' TATCGACGGT ACCGGCGGTG AGCAGATTGC AGAGTTCAAA G 3'
pf-calmo-KpnI-linker3: 5' TATCGACGGT ACCGGCGGTG GCGAGCAGAT TGCAGAGTTC AAAG 3'
pf-calmo-KpnI-linker4: 5' TATCGACGGT ACCGGCGGTG GCGGTGAGCA GATTGCAGAG TTCAAAG 3'
pf-calmo-KpnI-linker6: 5' TATCGACGGT ACCGGCGGTG GCTCTGGTGG CGAGCAGATT GCAGAGTTCA AAG 3'
pf-calmo-KpnI-linker9: 5' TATCGACGGT ACCGGCGGTG GCGGTTCTGG CGGTGGCGGT GAGCAGATTG CAGAGTTCAA AG 3'

reverse primers:

pr-calmo-SpeI-linker2: 5' AACGATCACT AGTACCTCCC TTTGCTGTCA TCATTTGTAC A 3'
pr-calmo-SpeI-linker3: 5' AACGATCACT AGTACCGCCA CCGCCCTTTG CTGTCATCAT TTGTACA 3'
pr-calmo-SpeI-linker4: 5' AACGATCACT AGTGCCACCG CCTCCCTTTG CTGTCATCAT TTGTACA 3'
pr-calmo-SpeI-linker6: 5' AACGATCACT AGTACCGCCA CCAGAGCCAC CCTTTGCTGT CATCATTTGT ACA 3'
pr-calmo-SpeI-linker9: 5' AACGATCACT AGTACCGCCA CCGCCAGAAC CGCCACCGCC CTTTGCTGTC ATCATTTGTA CA 3'