Melb:Plan:AND Gate

From 2007.igem.org

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Watch this space. There will be a plan here by Saturday night - Sorry it's not here yet
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[[Melbourne/Plan|<return to plans page>]]
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#Retransform c1 inverter (afternoon + overnight)
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#Liquid culture (overnight)
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#Isolate plasmid (mini prep kit not working - to big? - best way to isolate?) (half day)
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#Confirm Isolation and part using XbaI/PstI digest (half day)
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#*If feeling optimistic digest OmpR promoter simultaneously with SpeI/PstI and prepare to gel purify fragments of OmpR promoter (vector) and the inverter(insert)
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#If positive ligate (overnight)
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#Transform and liquid culture several colonies to confirm (1 afternoon + overnight + 15min + overnight)
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#Miniprep and digest to confirm and glycerol stocks. (1 day)
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Next need to ligate to RBS-ComA-Ter construct.

Latest revision as of 08:08, 11 August 2007

<return to plans page>

  1. Retransform c1 inverter (afternoon + overnight)
  2. Liquid culture (overnight)
  3. Isolate plasmid (mini prep kit not working - to big? - best way to isolate?) (half day)
  4. Confirm Isolation and part using XbaI/PstI digest (half day)
    • If feeling optimistic digest OmpR promoter simultaneously with SpeI/PstI and prepare to gel purify fragments of OmpR promoter (vector) and the inverter(insert)
  5. If positive ligate (overnight)
  6. Transform and liquid culture several colonies to confirm (1 afternoon + overnight + 15min + overnight)
  7. Miniprep and digest to confirm and glycerol stocks. (1 day)

Next need to ligate to RBS-ComA-Ter construct.