Melbourne/Lab Notebook Weeks 9-12

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Contents

Week 9

19 Aug 2007

  • Picked 3-4 transformants from "ligation" plates (17/8) and Grew up liquid cultures.
  • Transformed the two ligation reactions in the pink rack in the cold room and plated on Gen + Amp plate.


20 Aug 2007

Desired products:

    1. 450bp (95bp different from CI-2 used as control)
    2. 450bp
    3. 450bp
    4. 1kb
    5. 1kb
  • These were run on a gel.


21 Aug 2007

OmpR-inverter
-Vector: OmpR (AmpR) miniprepped P1 15P from 10/7 digested with S/P.
-Insert: c1 inverter (1kb fragment) P2 13K A with X/P.


RBS-LacZ
-Vector: LacZ (KanR) P2 9E from 6/8 with E/X.
-Insert: RBS (GenR) P4 8J from 6/7 with E/S.


Digested for 2h and heat inactivated for 10min at 80degC.


22 Aug 2007

  • Picked 4 colonies from fresh ligation/transformation to Growing up.

- These transformations were made as follows:

    • Ligated
      1. 5ul of Purified insert from 17/8 (CI-2 E/S)
      2. 5ul of Redigested P1 5G with E/X and heat inactivated at 60degC for 10min.


23 Aug 2007


24 Aug 2007


25 Aug 2007

Week 10

26 Aug 2007


27 Aug 2007


28 Aug 2007


29 Aug 2007


30 Aug 2007


31 Aug 2007


1 Sept 2007


Week 11

2 Sept 2007


3 Sept 2007


4 Sept 2007


5 Sept 2007


6 Sept 2007


7 Sept 2007


8 Sept 2007


Week 12

9 Sept 2007


10 Sept 2007


11 Sept 2007


12 Sept 2007


13 Sept 2007


14 Sept 2007


15 Sept 2007