Melbourne/Primary Reagents & disposables
From 2007.igem.org
(Difference between revisions)
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**[[Melbourne QuickchangeXL|QuickchangeII XL 200522(x30) 200521(x10): single change 2 primers 80% >8Kb]] | **[[Melbourne QuickchangeXL|QuickchangeII XL 200522(x30) 200521(x10): single change 2 primers 80% >8Kb]] | ||
*Primer design program : http://www.stratagene.com/sdmdesigner/default.aspx | *Primer design program : http://www.stratagene.com/sdmdesigner/default.aspx | ||
| - | + | ===Cells=== | |
| + | *[[Melbourne pBluescriptKS|pBluescriptKS+]] | ||
| + | *[[Melbourne DH5a|DH5a super competant cells]] | ||
===Enzymes=== | ===Enzymes=== | ||
*[[Melbourne/primary Restriction enzymes]] | *[[Melbourne/primary Restriction enzymes]] | ||
Revision as of 06:58, 12 July 2007
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Contents |
Primary Reagents
Kits
- IGEM 2007 kit
- Miniprep kit
- Gel clean up kit
- Site directed mutagenesis (stratagene)
- Quickchange multi 200515(x10),200514(x30): 3-5 changes 1 primer each 50% <8Kb
- Quickchange 200518(x30) 200519(x10): single change 2 primers 90% <8Kb
- QuickchangeII XL 200522(x30) 200521(x10): single change 2 primers 80% >8Kb
- Primer design program : http://www.stratagene.com/sdmdesigner/default.aspx
Cells
Enzymes
- Melbourne/primary Restriction enzymes
- T4 Ligase
- T4 Ligase buffer
- DH5a Competent cells
- DNA ladder
- phospatase
Antibiotics
Chemicals
Other
Disposables
- Parrafilm
- Tips (10, 200, 1000 ul)
- Gloves (Large , Med, Small)
- Scalpels
- Tube racks
- Freezer boxes
TUBES:
- Falcon: 10ml, 50ml
- microcentrifuge: 1.7ml
- PCR
