NYMU Taipei/Available Restriction Enzymes
From 2007.igem.org
Unit Definition of Restriction Enzymes
- One unit is defined as the amount of enzyme required to hydrolyze 1µg of λ DNA to completion in 60 minutes in a total reaction volume of 50 µl, under optimal assay conditions (salt concentration, pH, temperature).
- Some enzymes which do not, or rarely, cleave λ DNA. DNA are tested using another substrate; for example, enzymes such as Not I or Sfi I are tested with adenovirus DNA, and Fok I is tested using pBR322.
Setting Up a Restriction Endonuclease Reaction
Standard Restriction Enzyme Reactions
FAQs for Restriction Endonucleases
Restriction Modification System
- AatII
- AccI
- AciI
- AseI
- BamHI
- BanII
- BbsI
- BbvI
- BglII
- BsaBI
- BspEI
- EcoRI
- EcoRV
- HindIII
- MboI
- MspI
- NcoI
- NdeI
- NheI
- NotI
- PacI
- PstI
- RsaI
- SacI
- SalI
- SmaI
- SpeI
- SwaI
- XbaI
- XhoI
- XmaI
- XmnI