OtsA-construction file

(Difference between revisions)

Revision as of 17:49, 2 July 2007

PCR ThpA F/R on ThpA (553bp, BglII/XhoI)
Sub into PBca1144 (BglII/XhoI, 2054+910 L)
Product is pBca9145-BBa I716331

ThpAF Forward BglII site anneals to ThpA cgtacAGATCTatgaccgccgcaccagcagattttgcccgtgcccgaagcgaGttcctcagtatcg

ThpAR Reverse XhoI site anneals to ThpA anneals to XhoI cgcatctcgagtcaggtggcgaacgggccta

@@ Line 1: / Line 1: @@
-PCR otsAF/otsAIR on otsA     (413 bp, gp=A)
+PCR ThpA F/R on ThpA           (553bp, BglII/XhoI)<br>
-PCR otsAIF/otsAR on otsA     (1077 bp, gp=B)
+Sub into PBca1144                  (BglII/XhoI, 2054+910  L)<br>
---------------------------------------------
+Product is pBca9145-BBa I716331       <br>
-PCR otsAF/otsAR on A+B       (1456 bp BglII/XhoI)
+---------------------------------------------------------------------------------------------<br>
-Digest pBca9145-Bca1144      (BglII/XhoI 2054+910, L)
+ThpAF  Forward BglII site anneals to ThpA cgtacAGATCTatgaccgccgcaccagcagattttgcccgtgcccgaagcgaGttcctcagtatcg<br>
-Product pBca9145-BBa I716301
+<br>
---------------------------------------------
-otsAF forward BglII site anneals to otsA cgtaaAGATCTATGAGTCGTTTAGTCGTAGTATC
+ThpAR  Reverse XhoI site anneals to ThpA anneals to XhoI cgcatctcgagtcaggtggcgaacgggccta
-otsAIF Removing BamHI site in otsA CGATGACATTATCTGGATtCACGATTATCACCTG
-otsAIR Removing BamHI site in otsA CAGGTGATAATCGTGaATCCAGATAATGTCATCG
-otsaR reverse XhoI site anneals to otsA cgtatCTCGAGttaGGATCCTTACGCAAGCTTTGGAAAGGTAGC