Paris/July 22

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< Paris(Difference between revisions)
(Digestion products)
 
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[[Paris/July 21|yesterday]] -- [[Paris/July 23|tomorrow]] <br>
== Digestion products ==
== Digestion products ==
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elution of the purification products in 30-40microL H2O
elution of the purification products in 30-40microL H2O
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== ON cultures launched ==
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== Overnight cultures launched ==
The following ON cultures were launched:
The following ON cultures were launched:
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I was unable to launch the following culture because of lack of a glycerol stock:  
I was unable to launch the following culture because of lack of a glycerol stock:  
*pJ23107 clone 2 (from the glycerol stock S6.2) in 5ml LB-Amp. For performing a miniprep
*pJ23107 clone 2 (from the glycerol stock S6.2) in 5ml LB-Amp. For performing a miniprep
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== E.Coli pKS::DGAT ==
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We look under microscopy 72 hours after incubation of E.coli transformed by pKS::DGAT and the control E.coli transformed by part B0015  on different LB medium (See [[Paris/July_18#E.coli_pKS::DGAT|July 18]]).
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* Observation:
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We can observe E.coli single cells (100X).
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[[Image: coli_dgat_07222007.jpg|center|900px]]
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* Interpretation:
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We can observe lipid inclusion into E.coli transformed by pKS::DGAT with IPTG induction. With or without adding oleate we do not observe significant differences. Signal would be better with more cells.

Latest revision as of 17:49, 7 October 2007

yesterday -- tomorrow

Digestion products

Purification of the digestion products of 21/07/07 purif. using the SV wizard gel cleanup system

elution of the purification products in 30-40microL H2O

Overnight cultures launched

The following ON cultures were launched:

  • pJ23107 clone 3 (from the glycerol stock S14.3) in 5ml LB-Amp. For performing a miniprep
  • w121 (from glycerol stock S12) in 5 ml LB-Erythromycin. For performing growth-kinetics analysis on DAP supplemented media

I was unable to launch the following culture because of lack of a glycerol stock:

  • pJ23107 clone 2 (from the glycerol stock S6.2) in 5ml LB-Amp. For performing a miniprep

E.Coli pKS::DGAT

We look under microscopy 72 hours after incubation of E.coli transformed by pKS::DGAT and the control E.coli transformed by part B0015 on different LB medium (See July 18).

  • Observation:

We can observe E.coli single cells (100X).

Coli dgat 07222007.jpg


  • Interpretation:

We can observe lipid inclusion into E.coli transformed by pKS::DGAT with IPTG induction. With or without adding oleate we do not observe significant differences. Signal would be better with more cells.