Paris/July 3
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- | * Preparation of the P1 stock on the w121 strain. See [[Paris/PROTOCOLS|protocols]]. | + | * Preparation of the P1 stock on the w121 strain. See [[Paris/PROTOCOLS#Preparation of the P1 stock on the w121 strain.|protocols]]. |
* Kinetics measurements: | * Kinetics measurements: | ||
200ul LB: +0µl DAP, +2µl DAP, +4µl DAP, +8µl DAP, +12µl DAP <br> | 200ul LB: +0µl DAP, +2µl DAP, +4µl DAP, +8µl DAP, +12µl DAP <br> |
Revision as of 14:49, 10 July 2007
- Preparation of the P1 stock on the w121 strain. See protocols.
- Kinetics measurements:
200ul LB: +0µl DAP, +2µl DAP, +4µl DAP, +8µl DAP, +12µl DAP
200ul S0: +0µl DAP, +2µl DAP, +4µl DAP, +8µl DAP, +12µl DAP
S0= Centrifuged and filtered medium of MG1655 ON
DAP is 5mM