Paris/July 3
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| - | + | == Preparation of the P1 stock on the w121 strain. == | |
| - | + | See [[Paris/PROTOCOLS#Preparation of the P1 stock on the w121 strain.|protocols]]. | |
| - | + | This preparation is necessary for transduction of DapA deletion in MG1655 strain. | |
| - | + | == Kinetics measurements: == | |
| - | + | As previously described, we want to measure | |
| - | + | * Growth of DapA- strain (w121) relative to the concentration of DAP in the medium : | |
| - | + | 200µl LB: +0µl DAP, +2µl DAP, +4µl DAP, +8µl DAP, +12µl DAP | |
| + | * The excretion of Dap by MG1655 DapA+ strain by an indirect way : | ||
| + | We measure the growth of DapA- strain in the filtered growth medium of previously incubated MG1655 strain (S0) | ||
| + | S0 = Centrifuged and filtered medium of MG1655 ON <br> | ||
| + | 200µl S0: +0µl DAP, +2µl DAP, +4µl DAP, +8µl DAP, +12µl DAP | ||
| + | [DAP] = 5mM (''yes but what does it refer to ? [[User:Nicolas C.|Nicolas C.]])'' | ||
[[Paris|<<home]] | [[Paris|<<home]] | ||
Revision as of 15:00, 10 July 2007
Preparation of the P1 stock on the w121 strain.
See protocols. This preparation is necessary for transduction of DapA deletion in MG1655 strain.
Kinetics measurements:
As previously described, we want to measure
- Growth of DapA- strain (w121) relative to the concentration of DAP in the medium :
200µl LB: +0µl DAP, +2µl DAP, +4µl DAP, +8µl DAP, +12µl DAP
- The excretion of Dap by MG1655 DapA+ strain by an indirect way :
We measure the growth of DapA- strain in the filtered growth medium of previously incubated MG1655 strain (S0)
S0 = Centrifuged and filtered medium of MG1655 ON
200µl S0: +0µl DAP, +2µl DAP, +4µl DAP, +8µl DAP, +12µl DAP
[DAP] = 5mM (yes but what does it refer to ? Nicolas C.)
