Paris/July 3

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Preparation of the P1 stock on the w121 strain.

See protocols. This preparation is necessary for transduction of DapA deletion in MG1655 strain.

Kinetic measurements:

As previously described, we want to measure :

Growth of DapA- strain (w121) relative to the concentration of DAP in the medium :
The excretion of Dap by MG1655 DapA+ strain by an indirect way

Growth of DapA- strain (w121) relative to the concentration of DAP in the medium

We measure the kinetic of the w121 as a function of DAP concentration

Kinetic Array :Growthing of w121 strain as a function of DAP concentration (1µL w121 strain incubated ON)
	1	2	3	4	5	6	7	8	9	10	11	12
A											{{{A11}}}	{{{A12}}}
B		200µL LB + 0µL DAP	200µL LB + 2µL DAP	200µL LB + 4µL DAP	200µL LB + 8µL DAP	200µL LB + 12µL DAP					{{{B11}}}	{{{B12}}}
C											{{{C11}}}	{{{C12}}}
D											{{{D11}}}	{{{D12}}}
E											{{{E11}}}	{{{E12}}}
F											{{{F11}}}	{{{F12}}}
G											{{{G11}}}	{{{G12}}}
H	{{{H1}}}	{{{H2}}}	{{{H3}}}	{{{H4}}}	{{{H5}}}	{{{H6}}}	{{{H7}}}	{{{H8}}}	{{{H9}}}	{{{H10}}}	{{{H11}}}	{{{H12}}}

[DAP] = 5mM

The excretion of Dap by MG1655 DapA+ strain by an indirect way :

We measure the growth of DapA- strain in the filtered growth medium of previously incubated MG1655 strain (S0) S0 = Centrifuged and filtered medium of MG1655 ON

Kinetic Array :Growthing of w121 strain 'supplemented' with S0 (1µL w121 strain incubated ON)
	1	2	3	4	5	6	7	8	9	10	11	12
A											{{{A11}}}	{{{A12}}}
B		200µL S0 + 0µL DAP	200µL S0 + 2µL DAP	200µL S0 + 4µL DAP	200µL S0 + 8µL DAP	200µL S0 + 12µL DAP					{{{B11}}}	{{{B12}}}
C											{{{C11}}}	{{{C12}}}
D											{{{D11}}}	{{{D12}}}
E											{{{E11}}}	{{{E12}}}
F											{{{F11}}}	{{{F12}}}
G											{{{G11}}}	{{{G12}}}
H	{{{H1}}}	{{{H2}}}	{{{H3}}}	{{{H4}}}	{{{H5}}}	{{{H6}}}	{{{H7}}}	{{{H8}}}	{{{H9}}}	{{{H10}}}	{{{H11}}}	{{{H12}}}

[DAP] = 5mM

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@@ Line 7: / Line 7: @@
 * The excretion of Dap by MG1655 DapA+ strain by an indirect way
 === Growth of DapA- strain (w121) relative to the concentration of DAP in the medium ===
-We measure the kinetic of the w121 in an array <br>
+We measure the kinetic of the w121 as a function of DAP concentration <br>
 {{Paris_KineticArray| Title = Growthing of w121 strain as a function of DAP concentration (1µL w121 strain incubated ON)
 |A1=|A2=|A3=|A4=|A5=|A6=|A7=|A8=|A9=|A10=

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