From 2007.igem.org

Contents 1 Preparation of the P1 stock on the w121 strain. 2 Kinetics measurements: 2.1 Growth of DapA- strain (w121) relative to the concentration of DAP in the medium 2.2 The excretion of Dap by MG1655 DapA+ strain by an indirect way :

Preparation of the P1 stock on the w121 strain.

See protocols. This preparation is necessary for transduction of DapA deletion in MG1655 strain.

Kinetics measurements:

As previously described, we want to measure :

• Growth of DapA- strain (w121) relative to the concentration of DAP in the medium :
• The excretion of Dap by MG1655 DapA+ strain by an indirect way

Growth of DapA- strain (w121) relative to the concentration of DAP in the medium

We measure the kinetic of the w121 in an array
200µl LB: +0µl DAP, +2µl DAP, +4µl DAP, +8µl DAP, +12µl DAP

The excretion of Dap by MG1655 DapA+ strain by an indirect way :

We measure the growth of DapA- strain in the filtered growth medium of previously incubated MG1655 strain (S0) S0 = Centrifuged and filtered medium of MG1655 ON
200µl S0: +0µl DAP, +2µl DAP, +4µl DAP, +8µl DAP, +12µl DAP

[DAP] = 5mM (yes but what does it refer to ? Nicolas C.)

<<home