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Revision as of 12:53, 13 July 2007 (view source) Nicolas C. (Talk | contribs) ← Older edit		Revision as of 12:55, 13 July 2007 (view source) Nicolas C. (Talk | contribs) Newer edit →
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	|- style="background: #cccccc;"		|- style="background: #cccccc;"
	| style="background: #ccffcc;" | 11		| style="background: #ccffcc;" | 11
-	|	+	| Lox66-KmR-R
-	|	+	| TTTTACTAGTAGCGGCCGCTGCAGTACCGTTCGTATAATGTATGCTATACCAAGTTATTTATTAGAAAAACTCATCGAGCATCAAA
-	|	+	| 86
	|		|
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
	| style="background: #ccffcc;" | 12		| style="background: #ccffcc;" | 12
-	|	+	| DPst1-DGAT-F
-	|	+	| AGTGCTGTCGTATATGGCCCAGCAGGACTCAACATAATTT
-	|	+	| 40
	|		|
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
	| style="background: #ccffcc;" | 13		| style="background: #ccffcc;" | 13
-	|	+	| DPst1-DGAT-R
-	|	+	| AAATTATGTTGAGTCCTGCTGGGCCATATACGACAGCACT
-	|	+	| 40
	|		|
	|}		|}

---

Revision as of 12:55, 13 July 2007

Constructions

• Lox71-FtsZ:

Lox71-FtsZ-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT 89

FtsZ-R TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCAGGAAT 49

• Lox71-FtsA-FtsZ:

Lox71-FtsA-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAATCAGGCACAGGCAGAACAACAATG 89

• Deletion of Ecor1 site in FtsZ:

DEcoR1-FtsZ-R CGGTATTTACCGCGAAGAACTCAACACCTTCAATGCGCTC 40

DEcoR1-FtsZ-F TGCGCGAGCGCATTGAAGGTGTTGAGTTCTTCGCGGTAAA 40

• Lox66-DapAColi:

Lox66-DapAColi-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAGTTTGCACAGAGGATGGCCCATGTT 84

DapAColi-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGCAAACCGGCATGCTTAAGC 52

• Lox66-DapASubtilis:

Lox66-DapASubtilis-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAATCATGATGAGGTGAATAAAATGAA 84

DapASubtilis-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT 52

• Lox71-KmR-Lox66:

Lox71-KmR-F TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACGAAGTTATCTGATCCTTCAACTCAGCAAAAGTTC 85

Lox66-KmR-R TTTTACTAGTAGCGGCCGCTGCAGTACCGTTCGTATAATGTATGCTATACCAAGTTATTTATTAGAAAAACTCATCGAGCATCAAA 86

• Deletion of Pst1 site in DGAT:

DPst1-DGAT-F AGTGCTGTCGTATATGGCCCAGCAGGACTCAACATAATTT 40

DPst1-DGAT-R AAATTATGTTGAGTCCTGCTGGGCCATATACGACAGCACT 40

Table of Oligos
Number	Name	Sequence	Length	Comments
1	Lox71-FtsZ-F	TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT	89
2	FtsZ-R	TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCAGGAAT	49
3	Lox71-FtsA-F	TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAATCAGGCACAGGCAGAACAACAATG	89
4	DEcoR1-FtsZ-R	CGGTATTTACCGCGAAGAACTCAACACCTTCAATGCGCTC	40
5	DEcoR1-FtsZ-F	TGCGCGAGCGCATTGAAGGTGTTGAGTTCTTCGCGGTAAA	40
6	Lox66-DapAColi-F	TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAGTTTGCACAGAGGATGGCCCATGTT	84
7	DapAColi-R	TTTCTGCAGCGGCCGCTACTAGTATTATTACAGCAAACCGGCATGCTTAAGC	52
8	Lox66-DapASubtilis-F	TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAATCATGATGAGGTGAATAAAATGAA	84
9	DapASubtilis-R	TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT	52
10	Lox71-KmR-F	TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACGAAGTTATCTGATCCTTCAACTCAGCAAAAGTTC	85
11	Lox66-KmR-R	TTTTACTAGTAGCGGCCGCTGCAGTACCGTTCGTATAATGTATGCTATACCAAGTTATTTATTAGAAAAACTCATCGAGCATCAAA	86
12	DPst1-DGAT-F	AGTGCTGTCGTATATGGCCCAGCAGGACTCAACATAATTT	40
13	DPst1-DGAT-R	AAATTATGTTGAGTCCTGCTGGGCCATATACGACAGCACT	40