Paris/Oligos

From 2007.igem.org

(Difference between revisions)
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== Constructions ==
 
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* Lox71-FtsZ:
 
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Lox71-FtsZ-F      TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT 89     
 
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FtsZ-R                TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCAGGAAT 49
 
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* Lox71-FtsA-FtsZ:
 
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Lox71-FtsA-F          TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAATCAGGCACAGGCAGAACAACAATG 89     
 
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* Deletion of Ecor1 site in FtsZ:
 
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DEcoR1-FtsZ-R        CGGTATTTACCGCGAAGAACTCAACACCTTCAATGCGCTC 40
 
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DEcoR1-FtsZ-F        TGCGCGAGCGCATTGAAGGTGTTGAGTTCTTCGCGGTAAA 40
 
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* Lox66-DapAColi:
 
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Lox66-DapAColi-F      TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAGTTTGCACAGAGGATGGCCCATGTT 84   
 
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DapAColi-R            TTTCTGCAGCGGCCGCTACTAGTATTATTACAGCAAACCGGCATGCTTAAGC 52
 
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* Lox66-DapASubtilis:
 
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Lox66-DapASubtilis-F  TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAATCATGATGAGGTGAATAAAATGAA 84     
 
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DapASubtilis-R        TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT 52
 
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* Lox71-KmR-Lox66:
 
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Lox71-KmR-F          TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACGAAGTTATCTGATCCTTCAACTCAGCAAAAGTTC 85 
 
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Lox66-KmR-R          TTTTACTAGTAGCGGCCGCTGCAGTACCGTTCGTATAATGTATGCTATACCAAGTTATTTATTAGAAAAACTCATCGAGCATCAAA 86
 
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* Deletion of Pst1 site in DGAT:
 
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DPst1-DGAT-F          AGTGCTGTCGTATATGGCCCAGCAGGACTCAACATAATTT 40
 
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DPst1-DGAT-R          AAATTATGTTGAGTCCTGCTGGGCCATATACGACAGCACT 40
 
{|
{|
|- style="background: #ccccff;"
|- style="background: #ccccff;"
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|width=25%| Comments
|width=25%| Comments
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
-
| style="background: #ccffcc;" | 1
+
| style="background: #ffcccc;" | 1
-
| Lox71-FtsZ-F
+
| style="background: #ffcccc;" |Lox71-FtsZ-F
-
| TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT
+
| style="background: #ffcccc;" |TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACG AAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT
-
| 89
+
| style="background: #ffcccc;" |89
-
|  
+
| style="background: #ffcccc;" |
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | 2
| style="background: #ccffcc;" | 2
Line 68: Line 23:
| style="background: #ccffcc;" | 3
| style="background: #ccffcc;" | 3
| Lox71-FtsA-F
| Lox71-FtsA-F
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| TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAATCAGGCACAGGCAGAACAACAATG
+
| TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACG AAGTTATTAATAATCAGGCACAGGCAGAACAACAATG
| 89
| 89
|  
|  
Line 86: Line 41:
| style="background: #ccffcc;" | 6
| style="background: #ccffcc;" | 6
| Lox66-DapAColi-F
| Lox66-DapAColi-F
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| TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAGTTTGCACAGAGGATGGCCCATGTT
+
| TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAGTTTGCACAGAGGATGGCCCATGTT
| 84
| 84
|
|
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| style="background: #ccffcc;" | 8
| style="background: #ccffcc;" | 8
| Lox66-DapASubtilis-F   
| Lox66-DapASubtilis-F   
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| TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAATCATGATGAGGTGAATAAAATGAA
+
| TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAATCATGATGAGGTGAATAAAATGAA
| 84
| 84
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
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| style="background: #ccffcc;" | 9
+
| style="background: #ffcccc;" | 9
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| DapASubtilis-R         
+
| style="background: #ffcccc;" |DapASubtilis-R         
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| TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT
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| style="background: #ffcccc;" |TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT
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| 52
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| style="background: #ffcccc;" |52
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|
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| style="background: #ffcccc;" |
|- style="background: #cccccc;"
|- style="background: #cccccc;"
| style="background: #ccffcc;" | 10
| style="background: #ccffcc;" | 10
| Lox71-KmR-F           
| Lox71-KmR-F           
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| TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACGAAGTTATCTGATCCTTCAACTCAGCAAAAGTTC
+
| TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACG AAGTTATCTGATCCTTCAACTCAGCAAAAGTTC
| 85
| 85
|
|
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| style="background: #ccffcc;" | 11
| style="background: #ccffcc;" | 11
| Lox66-KmR-R           
| Lox66-KmR-R           
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| TTTTACTAGTAGCGGCCGCTGCAGTACCGTTCGTATAATGTATGCTATACCAAGTTATTTATTAGAAAAACTCATCGAGCATCAAA
+
| TTTTACTAGTAGCGGCCGCTGCAGTACCGTTCGTATAATGTATGCTATACCA AGTTATTTATTAGAAAAACTCATCGAGCATCAAA
| 86
| 86
|
|

Revision as of 13:01, 13 July 2007

Table of Oligos
Number Name Sequence Length Comments
1 Lox71-FtsZ-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACG AAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT 89
2 FtsZ-R TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCAGGAAT 49
3 Lox71-FtsA-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACG AAGTTATTAATAATCAGGCACAGGCAGAACAACAATG 89
4 DEcoR1-FtsZ-R CGGTATTTACCGCGAAGAACTCAACACCTTCAATGCGCTC 40
5 DEcoR1-FtsZ-F TGCGCGAGCGCATTGAAGGTGTTGAGTTCTTCGCGGTAAA 40
6 Lox66-DapAColi-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAGTTTGCACAGAGGATGGCCCATGTT 84
7 DapAColi-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGCAAACCGGCATGCTTAAGC 52
8 Lox66-DapASubtilis-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAATCATGATGAGGTGAATAAAATGAA 84
9 DapASubtilis-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT 52
10 Lox71-KmR-F TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACG AAGTTATCTGATCCTTCAACTCAGCAAAAGTTC 85
11 Lox66-KmR-R TTTTACTAGTAGCGGCCGCTGCAGTACCGTTCGTATAATGTATGCTATACCA AGTTATTTATTAGAAAAACTCATCGAGCATCAAA 86
12 DPst1-DGAT-F AGTGCTGTCGTATATGGCCCAGCAGGACTCAACATAATTT 40
13 DPst1-DGAT-R AAATTATGTTGAGTCCTGCTGGGCCATATACGACAGCACT 40
Retrieved from "http://2007.igem.org/wiki/index.php/Paris/Oligos"