Tokyo/Genetic circuit
From 2007.igem.org
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In the A-state cell, promoter Pa is "on", and the cell expresses repressor Ra, luxI, and RFP. Repressor Ra put the promoter Pb into "off", and prohibits to change the state. | In the A-state cell, promoter Pa is "on", and the cell expresses repressor Ra, luxI, and RFP. Repressor Ra put the promoter Pb into "off", and prohibits to change the state. | ||
This cell also expresses luxI, which makes AHL (cell-cell communication molecule). And in the downstream of these genes, there is an RFP gene. So we can detect state A cell using FACS. | This cell also expresses luxI, which makes AHL (cell-cell communication molecule). And in the downstream of these genes, there is an RFP gene. So we can detect state A cell using FACS. | ||
Revision as of 16:03, 23 October 2007
Abstract Concept & Model Requirements Genetic_circuit Works About_our_team
Genetic Circuit
Fig1 is an outline of our genetic circuit, designed for realizing our Concept&Model.
All the cells (Wokers and Idlers) have the same genetic circuit. (Just as the 'society' members have practically the same genetic backgrounds)
Worker (State-A)
(Fig2) In the A-state cell, promoter Pa is "on", and the cell expresses repressor Ra, luxI, and RFP. Repressor Ra put the promoter Pb into "off", and prohibits to change the state. This cell also expresses luxI, which makes AHL (cell-cell communication molecule). And in the downstream of these genes, there is an RFP gene. So we can detect state A cell using FACS.
Idler
Idlerとして機能している場合、
Worker pick up
