Tokyo/Genetic circuit

From 2007.igem.org

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==Idlers (State-B)==
==Idlers (State-B)==
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'''Fig. 3''' shows a B-state cell, in which promoter Pb is "turned on" in the presence of AHL, and repressor Rb and GFP are expressed. Repressor Rb represses the expression of promoter Pa and thereby mcherry; therefore, fluorescence of GFP is used to detect state-B cells.
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'''Fig. 3''' shows a B-state cell, in which promoter Pb is "turned on" in the presence of AHL, and repressor Rb and GFP are expressed. Repressor Rb represses the expression of promoter Pa and thereby RFP; therefore, fluorescence of GFP is used to detect state-B cells.
==If Workers are removed...==
==If Workers are removed...==

Revision as of 12:18, 24 October 2007


Abstract  Concept & Model  Requirements  Genetic_circuit  Works  About_our_team

Genetic Circuit

Fig.1 is an outline of the genetic circuit in our model, designed to realize our Concept & Model.

All cells, wokers and idlers, have THE SAME genetic circuit (just as 'society' members have practically the same genetic backgrounds).

Fig.1: Genetic Circuit

Workers (State-A)

As shown in Fig. 2, an A-state cell, in which the promoter Pa is "on," expresses the repressor Ra, luxI, and RFP. The repressor Ra "turns off" the promoter Pb and prevents from changing the cell states. This cell also expresses luxI, which makes AHL, cell-cell communication molecules. Downstream of these genes, there is an RFP gene; therefore, we would be able to detect state-A cell with FACS.

Fig.2: Workers (State-A)

Idlers (State-B)

Fig. 3 shows a B-state cell, in which promoter Pb is "turned on" in the presence of AHL, and repressor Rb and GFP are expressed. Repressor Rb represses the expression of promoter Pa and thereby RFP; therefore, fluorescence of GFP is used to detect state-B cells.

If Workers are removed...

(Fig 4)If A-state cells are removed, B-state cells recognizes the absense of A-cells. That is, they respond to the decrease of AHL, not to produce repressor Rb. In this condition, repressions of Pa by Rb, and Pb by Ra are declined. This puts the toggle-like circuit into "reset".
(鮎川)toggle-likeという言葉はうちのgenetic circuitには当てはまらないと思います。そもそもtoggle switchにAでもBでもないresetという状態はないのでは?

Fig.3: Idlers (State-B)
Fig.4: If Workers are removed...

Hybrid promoter

これを実装するためにPbはハイブリッドプロモータである必要がある。 ⇒ハイブリッドプロモーターとは?WorkのページへHybrid promoter

他の遺伝子回路の案

コンセプトモデルを遺伝子回路にする上で、他にもいくつか方法が考えられた。 その案を以下に示す。