Tokyo/Works
From 2007.igem.org
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We have alternated/combined Wet and Dry experiments to achieve our goal | We have alternated/combined Wet and Dry experiments to achieve our goal | ||
| - | + | ==0.<span style="color:#ff33cc;">Wet </span>: [[Tokyo/Hybrid promoter|Hybrid promoter]]== | |
{| cellspacing="5" | {| cellspacing="5" | ||
|width="350px" valign="top" | | |width="350px" valign="top" | | ||
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| - | + | ==1.<span style="color:#0066ff;">Dry </span>: [[Tokyo/Formulation |Formulation]]== | |
We wrote the Formulation | We wrote the Formulation | ||
<!--リプレッサーＡ，Ｂ，シグナル分子Ｓの時間変化を式として表し、安定性解析を行った結果、Hill係数が大切であることがわかった | <!--リプレッサーＡ，Ｂ，シグナル分子Ｓの時間変化を式として表し、安定性解析を行った結果、Hill係数が大切であることがわかった | ||
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--> | --> | ||
| - | + | ==2.<span style="color:#ff33cc;">Wet </span>: [[Tokyo/Assay |Assay]]== | |
====We decided the parameter of hill coefficients by the assay of AHL & IPTG==== | ====We decided the parameter of hill coefficients by the assay of AHL & IPTG==== | ||
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[[Image:hill AHL.JPG|190px]]|[[Image:hill IPTG.JPG|190px]] | [[Image:hill AHL.JPG|190px]]|[[Image:hill IPTG.JPG|190px]] | ||
| - | + | ==3.<span style="color:#0066ff;">Dry </span>: [[Tokyo/Simulation |Simulation]]== | |
wet実験で求められたパラメータを用いて、集団が怠け者の細胞のみとなった場合に、一度不安定化して、続いて働き者と怠け者になれることをStocastic Simulationで確認した。このシステムの挙動に必要な他のパラメータ範囲が判明した。 | wet実験で求められたパラメータを用いて、集団が怠け者の細胞のみとなった場合に、一度不安定化して、続いて働き者と怠け者になれることをStocastic Simulationで確認した。このシステムの挙動に必要な他のパラメータ範囲が判明した。 | ||
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<br>[[Image:Tokyo_simulation_s2.jpg|350px]] | <br>[[Image:Tokyo_simulation_s2.jpg|350px]] | ||
| - | + | ==4.<span style="color:#ff33cc;">Wet </span>: [[Tokyo/Assay |Assay]]== | |
<!--====We determined the feasibility test of this ====--> | <!--====We determined the feasibility test of this ====--> | ||
Simulationを満たすための他のパラメータが現在のプラスミドで可能であるかを、大腸菌を用いた実験で確認した。 | Simulationを満たすための他のパラメータが現在のプラスミドで可能であるかを、大腸菌を用いた実験で確認した。 | ||
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2. We tested whether our plasmids gave the other parameters to satisfy the simulation by the experiments using ''E. coli''. | 2. We tested whether our plasmids gave the other parameters to satisfy the simulation by the experiments using ''E. coli''. | ||
| - | + | ==5.Future works== | |
Based on the results from the wet experiments in the step 4, the next dry approach - analysis and simulation - should be performed, which will in turn be testified and confirmed by wet experiments. | Based on the results from the wet experiments in the step 4, the next dry approach - analysis and simulation - should be performed, which will in turn be testified and confirmed by wet experiments. | ||
Revision as of 18:31, 22 October 2007
1.Concept & Model 2.Requirements 3.Genetic circuit 4.Works
Works
Approach
Combination of Wet & Dry trials
We have alternated/combined Wet and Dry experiments to achieve our goal
0.Wet : Hybrid promoter
We need to develop the promoter that can receive two input, AHL & reperssor. We tried to create and succeeded!! |
|
1.Dry : Formulation
We wrote the Formulation
2.Wet : Assay
We decided the parameter of hill coefficients by the assay of AHL & IPTG
|
3.Dry : Simulation
wet実験で求められたパラメータを用いて、集団が怠け者の細胞のみとなった場合に、一度不安定化して、続いて働き者と怠け者になれることをStocastic Simulationで確認した。このシステムの挙動に必要な他のパラメータ範囲が判明した。
Based on stochastic simulation using parameters obtained from our wet experiments, we have confirmed that the whole system becomes unstable when there are only idlers left, and then, they become either of workers and idlers. Also we have determined other parameters necessary for the desired behavior of this system.
4.Wet : Assay
Simulationを満たすための他のパラメータが現在のプラスミドで可能であるかを、大腸菌を用いた実験で確認した。
1. By the wet experiments using E. coli, we testified whether our current plasmids could satisfy the other required conditions (for the desired behavior of the system) estimated by the simulation.
2. We tested whether our plasmids gave the other parameters to satisfy the simulation by the experiments using E. coli.
5.Future works
Based on the results from the wet experiments in the step 4, the next dry approach - analysis and simulation - should be performed, which will in turn be testified and confirmed by wet experiments. Continueing these processes, if time permits, would further sophisticate our model.
