Tokyo/Works

From 2007.igem.org

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  0. [[Tokyo/Works/Hybrid promoter|Hybrid promoter]]  1. [[Tokyo/Works/Formulation |Formulation]]  2. [[Tokyo/Works/Assay |Assay1]]  3. [[Tokyo/Works/Simulation |Simulation]]  4. [[Tokyo/Works/Assay2 |Assay2]]  5. [[Tokyo/Works/Future works |Future works]]
  0. [[Tokyo/Works/Hybrid promoter|Hybrid promoter]]  1. [[Tokyo/Works/Formulation |Formulation]]  2. [[Tokyo/Works/Assay |Assay1]]  3. [[Tokyo/Works/Simulation |Simulation]]  4. [[Tokyo/Works/Assay2 |Assay2]]  5. [[Tokyo/Works/Future works |Future works]]
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以下Worksのアブストとなる文章に。上下との話のつながりも明記。
以下Worksのアブストとなる文章に。上下との話のつながりも明記。
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==0. <span style="color:#ff33cc;">Wet </span>: [[Tokyo/Works/Hybrid promoter|Hybrid promoter]]==
==0. <span style="color:#ff33cc;">Wet </span>: [[Tokyo/Works/Hybrid promoter|Hybrid promoter]]==
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First, the newly deviced promoter sensed the '''Two inputs''' is necessary for the [[Tokyo/Genetic circuit|Genetic_circuit]].
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We made hybrid promoter
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The newly deviced promoter sensed the '''TWO INPUTS''', AHL & reperssor, as shown in Fig., working as an AND gate.
 
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<!--New part development:Hybrid promoterPBとして、2つの入力を受けるプロモータを開発する必要があった
 
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<br>図 概念図、配列とグラフ図
 
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[[Image:Hybridgraph.JPG|200px|]]
[[Image:Hybridgraph.JPG|200px|]]

Revision as of 06:57, 24 October 2007


Abstract  Concept & Model  Requirements  Genetic_circuit  Works  About_our_team

  0. Hybrid promoter  1. Formulation  2. Assay1  3. Simulation  4. Assay2  5. Future works

以下Worksのアブストとなる文章に。上下との話のつながりも明記。

Approach

We have alternated/combined Wet and Dry experiments to achieve our goal

0. Wet : Hybrid promoter

First, the newly deviced promoter sensed the Two inputs is necessary for the Genetic_circuit. We made hybrid promoter

Hybridgraph.JPG

1.Dry :  Formulation

簡単な式からわかることを導きながら、段階を経て今回必要な(←今回の遺伝子回路を実装するような)計算式を導出した。最終的な式では、確率微分方程式を用いている。 From simple equations, the equations necessary for our model have been deviated step by step. In the final step a stochastic differential equation is used.
Expression4-1.jpg

2.Wet :  Assay1 using "Externally added" materials

We decided the parameter of hill coefficients by the assay of AHL & IPTG
Hill AHL.JPG|Hill IPTG.JPG

3.Dry :  Simulation

Based on stochastic simulation using parameters obtained from our wet experiments, we have confirmed that the whole system becomes unstable when there are only idlers left, and then, they become either of workers and idlers. Also we have determined other parameters necessary for the desired behavior of this system.

Tokyo simulation s2.jpg

4.Wet :  Assay2 using "Cell-produced" AHL

By the wet experiments using E. coli, we testified whether our current plasmids can satisfy the other required conditions (for the desired behavior of the system) estimated by the simulation.

5.Future works

Based on the results from the wet experiments in the step 4, the next dry approach - analysis and simulation - should be performed, which will in turn be testified and confirmed by wet experiments. Continueing these processes, if time permits, would further sophisticate our model.