Tokyo/Works

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Abstruct  Concept & Model  Requirements  Genetic_circuit  Works  About_our_team


Works top  0.Hybrid promoter  1.Formulation  2.Assay  3.Simulation  4.Assay2  5.Future works

Works

Approach

Combination of Wet & Dry trials

We have alternated/combined Wet and Dry experiments to achieve our goal

0.Wet : Hybrid promoter

We need to develop the promoter that can receive two input, AHL & reperssor. We tried to create and succeeded!!

Hybridgraph.JPG

1.Dry :  Formulation

We wrote the Formulation

2.Wet :  Assay

We decided the parameter of hill coefficients by the assay of AHL & IPTG

Hill AHL.JPG|Hill IPTG.JPG

3.Dry :  Simulation

Based on stochastic simulation using parameters obtained from our wet experiments, we have confirmed that the whole system becomes unstable when there are only idlers left, and then, they become either of workers and idlers. Also we have determined other parameters necessary for the desired behavior of this system.

Tokyo simulation s2.jpg

4.Wet :  Assay using Cell-produced AHL

By the wet experiments using E. coli, we testified whether our current plasmids could satisfy the other required conditions (for the desired behavior of the system) estimated by the simulation.

5.Future works

Based on the results from the wet experiments in the step 4, the next dry approach - analysis and simulation - should be performed, which will in turn be testified and confirmed by wet experiments. Continueing these processes, if time permits, would further sophisticate our model.