Tokyo/Works/Assay
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| - | + | =[[Tokyo/Hill function fitting |Purpose of this assay (Hill function fitting) ]] = | |
新しく作ったハイブリッドプロモーターに関してはデータがないので，実験によりパラメータを求める． | 新しく作ったハイブリッドプロモーターに関してはデータがないので，実験によりパラメータを求める． | ||
ヒル関数をfittingさせることにより，n2,n3,K2,K3が求まる. | ヒル関数をfittingさせることにより，n2,n3,K2,K3が求まる. | ||
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| - | + | == [[Tokyo/Repression and activation check with LacI and AHL|1.AHL assay]] == | |
====Purpose==== | ====Purpose==== | ||
<br>check if AHL activates lux-lac hybrid promoter | <br>check if AHL activates lux-lac hybrid promoter | ||
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<br>[[Image:hill AHL.JPG|190px]] | <br>[[Image:hill AHL.JPG|190px]] | ||
| - | + | == [[Tokyo/IPTG assay|2.IPTG assay]] == | |
====Purpose==== | ====Purpose==== | ||
<br>To determine the order of the concentration of IPTG necessary for the activation of our lux-lac hybrid promoter in the LacI producing pTrc99A cells. | <br>To determine the order of the concentration of IPTG necessary for the activation of our lux-lac hybrid promoter in the LacI producing pTrc99A cells. | ||
Revision as of 04:40, 24 October 2007
Works top 0.Hybrid promoter 1.Formulation 2.Assay1 3.Simulation 4.Assay2 5.Future works
Purpose of this assay (Hill function fitting)
新しく作ったハイブリッドプロモーターに関してはデータがないので，実験によりパラメータを求める．
ヒル関数をfittingさせることにより，n2,n3,K2,K3が求まる.
We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.
1.AHL assay
Purpose
check if AHL activates lux-lac hybrid promoter
check if lacI represses lux-lac hybrid promoter
Result & Conclusion
AND gate by AHL & IPTG
Lux-lac hybrid promoter is activated only in the presence of AHL and IPTG.
2.IPTG assay
Purpose
To determine the order of the concentration of IPTG necessary for the activation of our lux-lac hybrid promoter in the LacI producing pTrc99A cells.
The order of the concentration is used for more detailed assay with narrower range of the IPTG concentration.
Result & Conclusion
2.Preliminary assays
We conducted preliminary assays to practice experiments and check if the materials and methods worked properly.
