Tokyo/Works/Assay

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(Difference between revisions)
(Purpose)
(Result & Conclusion)
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====Result & Conclusion====
====Result & Conclusion====
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<br>Lux-lac hybrid promoter is activated in the presence of AHL.
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<br>Lux-lac hybrid promoter is activated by AHL.
<br>[[Image:hill AHL.JPG|190px]]
<br>[[Image:hill AHL.JPG|190px]]

Revision as of 04:57, 24 October 2007


Works top  0.Hybrid promoter  1.Formulation  2.Assay1  3.Simulation  4.Assay2  5.Future works


Purpose of this assay (Hill function fitting)

新しく作ったハイブリッドプロモーターに関してはデータがないので,実験によりパラメータを求める. ヒル関数をfittingさせることにより,n2,n3,K2,K3が求まる. We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.
Expression6-1.JPG
Expression6-2.JPG



1.AHL assay

Purpose


check if AHL activates lux-lac hybrid promoter.
These parameters(n2,K2) is decided.

Result & Conclusion


Lux-lac hybrid promoter is activated by AHL.
Hill AHL.JPG

2.IPTG assay

Purpose


check if LacI represses this hybrid promoter indirectly-measured by IPTG.
These parameters(n3,K3) is decided.

Result & Conclusion


Hill IPTG.JPG

2.Preliminary assays

We conducted preliminary assays to practice experiments and check if the materials and methods worked properly.

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