Tokyo/Works/Assay
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== [[Tokyo/Repression and activation check with LacI and AHL|1.AHL assay]] == | == [[Tokyo/Repression and activation check with LacI and AHL|1.AHL assay]] == | ||
====Purpose==== | ====Purpose==== | ||
| - | + | check if AHL activates lux-lac hybrid promoter. | |
<br>These parameters(n2,K2) is decided. | <br>These parameters(n2,K2) is decided. | ||
Revision as of 04:57, 24 October 2007
Works top 0.Hybrid promoter 1.Formulation 2.Assay1 3.Simulation 4.Assay2 5.Future works
Purpose of this assay (Hill function fitting)
新しく作ったハイブリッドプロモーターに関してはデータがないので，実験によりパラメータを求める．
ヒル関数をfittingさせることにより，n2,n3,K2,K3が求まる.
We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.
1.AHL assay
Purpose
check if AHL activates lux-lac hybrid promoter.
These parameters(n2,K2) is decided.
Result & Conclusion
Lux-lac hybrid promoter is activated by AHL.
2.IPTG assay
Purpose
check if LacI represses this hybrid promoter indirectly-measured by IPTG.
These parameters(n3,K3) is decided.
Result & Conclusion
Lux-lac hybrid promoter is activated by AHL.
2.Preliminary assays
We conducted preliminary assays to practice experiments and check if the materials and methods worked properly.
