Tokyo/Works/Assay

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==Preliminary assays==
==Preliminary assays==

Revision as of 08:18, 24 October 2007


Works top  0.Hybrid promoter  1.Formulation  2.Assay1  3.Simulation  4.Assay2  5.Future works


Purpose of this assay (Hill function fitting)

新しく作ったハイブリッドプロモーターに関してはデータがないので,実験によりパラメータを求める. ヒル関数をfittingさせることにより,n2,n3,K2,K3が求まる. We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.
Expression6-1.JPG
Expression6-2.JPG



1.AHL assay

Purpose

check how AHL activates lux-lac hybrid promoter.
These parameters(n2,K2) is decided.

Result & Conclusion

Lux-lac hybrid promoter is activated by AHL as shown in fig X (fig番号).
Hill AHL.JPG

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2.IPTG assay

Purpose

check how LacI represses this hybrid promoter. In order to adjust LacI concentration, IPTG was added.
These parameters(n3,K3) is decided.

Result & Conclusion

Lux-lac hybrid promoter is activated by AHL.
Hill IPTG.JPG


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Preliminary assays

We conducted preliminary assays to practice experiments and check if the materials and methods worked properly.

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