Tokyo/Works/Assay
From 2007.igem.org
(Difference between revisions)
| Line 9: | Line 9: | ||
ヒル関数をfittingさせることにより，n2,n3,K2,K3が求まる.--> | ヒル関数をfittingさせることにより，n2,n3,K2,K3が求まる.--> | ||
We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3. | We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3. | ||
| - | <br>[[Image:expression6- | + | <br>[[Image:expression6-10-2.JPG|300px|]] |
| - | <br>[[Image:expression6- | + | <br>[[Image:expression6-11.JPG|340px|]] |
| - | + | ||
| - | + | ||
| - | + | ||
<!--== Assays for hybrid promoter == | <!--== Assays for hybrid promoter == | ||
Revision as of 02:42, 25 October 2007
Abstract Concept & Model Requirements Genetic_circuit Works About_our_team
Works top 0.Hybrid promoter 1.Formulation 2.Assay1 3.Simulation 4.Assay2 5.Future works
Purpose of this assay (Hill function fitting)
We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.
1.AHL assay
Purpose
check how AHL activates lux-lac hybrid promoter.
These parameters(n2,K2) is decided.
Result & Conclusion
Lux-lac hybrid promoter is activated by AHL as shown in fig X （fig番号）.
⇒see more details
2.IPTG assay
Purpose
check how LacI represses this hybrid promoter. In order to adjust LacI concentration, IPTG was added.
These parameters(n3,K3) is decided.
Result & Conclusion
Lux-lac hybrid promoter is activated by AHL.
⇒see more details
Appendix:Preliminary assays
We conducted preliminary assays to practice experiments and check if the materials and methods worked properly.
