Tokyo/Works/Assay

From 2007.igem.org

< Tokyo/Works
Revision as of 04:32, 24 October 2007 by Nakatani (Talk | contribs)


Works top  0.Hybrid promoter  1.Formulation  2.Assay1  3.Simulation  4.Assay2  5.Future works


Hill function fitting

新しく作ったハイブリッドプロモーターに関してはデータがないので,実験によりパラメータを求める. ヒル関数をfittingさせることにより,n2,n3,K2,K3が求まる. We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.
Expression6-1.JPG
Expression6-2.JPG



Repression and activation check with LacI and AHL

Purpose


check if AHL activates lux-lac hybrid promoter
check if lacI represses lux-lac hybrid promoter

Result & Conclusion


AND gate by AHL & IPTG
Lux-lac hybrid promoter is activated only in the presence of AHL and IPTG.
Hill AHL.JPG

IPTG assay

Purpose


To determine the order of the concentration of IPTG necessary for the activation of our lux-lac hybrid promoter in the LacI producing pTrc99A cells.
The order of the concentration is used for more detailed assay with narrower range of the IPTG concentration.

Result & Conclusion


Hill IPTG.JPG

2.Preliminary assays

We conducted preliminary assays to practice experiments and check if the materials and methods worked properly.

See more details