Tokyo/sunaba2

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<h1> ''' 1,[[Tokyo/Project |Project]]''' </h1>
<h1> ''' 1,[[Tokyo/Project |Project]]''' </h1>
===[[Tokyo/Concept |Concept & Model]]===
===[[Tokyo/Concept |Concept & Model]]===
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====Bacterial society that follows Pareto's principle====
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====We made bacterial society that follows Pareto's principle,for example ,[[Tokyo/Ant society|Ant society]]====
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<br>[[Image:Ant society.JPG|350px]]
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<br>[[Image:TOP.JPG|350px]]
<br>[[Image:TOP.JPG|350px]]
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===[[Tokyo/Requirements |Requirements]]: bistability and cell-cell communication===
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===[[Tokyo/Requirements |Requirements]]===
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====Our requirements for construction this model are two things,bistability and coexistence by cell-cell communication====
<br>[[Image:Bistability.JPG|200px]][[Image:Coexistence.JPG|250px]]
<br>[[Image:Bistability.JPG|200px]][[Image:Coexistence.JPG|250px]]

Revision as of 16:35, 21 October 2007

砂場2 Tokyotech3.JPG

1,Project

Concept & Model

We made bacterial society that follows Pareto's principle,for example ,Ant society


TOP.JPG

Requirements

Our requirements for construction this model are two things,bistability and coexistence by cell-cell communication


Bistability.JPGCoexistence.JPG

Genetic circuit:Quorum Sensing


Tokyo genetic circuit s.jpg

Our construction parts are Here!!!

2,Works

Approach:We alternated approach of Wet and Dry.

2-0.Wet : Hybrid promoter

We need to develop the promoter that can receive two input,AHL & reperssor.We tryed to create and successed!!

Hybrid.JPG

2-1.Dry : Formulation

We writed the Formulation

2-2.Wet : Assay

We decided the parameter of hill coefficients by the assay of AHL & IPTG

Hill AHL.JPG|Hill IPTG.JPG

2-3.Dry : Simulation

wet実験で求められたパラメータを用いて、集団が怠け者の細胞のみとなった場合に、一度不安定化して、続いて働き者と怠け者になれることをStocastic Simulationで確認した。このシステムの挙動に必要な他のパラメータ範囲が判明した。

Tokyo simulation s2.jpg

2-4.Wet : Assay

Simulationを満たすための他のパラメータが現在のプラスミドで可能であるかを、大腸菌を用いた実験で確認した。

2-5.Future works

3,About our team


Students
Satoru Akama | Kenichiro Iwasaki | Hiroki Kawahara | Hajime Nakatani

Advisors: Last year's member
Syotarou Ayukawa | Akio Kobayashi

Instructors
[http://www.sb.dis.titech.ac.jp/ Daisuke Kiga] | [http://www.es.dis.titech.ac.jp/ Masayuki Yamamura] | [http://nicosia.is.s.u-tokyo.ac.jp/~hagiya/ Masami Hagiya] | [http://www.sb.dis.titech.ac.jp/ Masahiko Uchiyama]

Links sunaba