Tokyo/sunaba2

From 2007.igem.org

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===[[Tokyo/Genetic circuit|Genetic circuit]]===
===[[Tokyo/Genetic circuit|Genetic circuit]]===
====We constructed the genetic circuit to fill its requirements. Therefore we made [[Tokyo/new parts |new parts]] ====
====We constructed the genetic circuit to fill its requirements. Therefore we made [[Tokyo/new parts |new parts]] ====
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<br>[[Image:Tokyo_genetic circuit_s.jpg|350px]]
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<br>[[Image:Tokyo_genetic circuit_s.jpg|450px]]
====               Our construction parts are [[Tokyo/construction|Here]]!!! ====
====               Our construction parts are [[Tokyo/construction|Here]]!!! ====

Revision as of 13:07, 22 October 2007

砂場2 Tokyotech3.JPG

1,Project

Concept & Model

We made bacterial society that follows Pareto's principle,for example ,Ant society


TOP.JPG

Requirements

Our requirements for construction this model are two things,bistability and coexistence by cell-cell communication


Bistability.JPGCoexistence.JPG

Genetic circuit

We constructed the genetic circuit to fill its requirements. Therefore we made new parts


Tokyo genetic circuit s.jpg

               Our construction parts are Here!!!

Our GOAL

We indicated our goal of the project by the simulation!! The simulation proved that our genetic circuit have existence of solution.


Tokyo simulation s2.jpg

Result

We indicated our goal of the project by the simulation!! The simulation proved that our genetic circuit have existence of solution.


Tokyo simulation s2.jpg

2,Works


2-0.Wet : Hybrid promoter

Hybridgraph.JPG

2-1.Dry : Formulation

Expression4-1.jpg

2-2.Wet : Assay

Hill AHL.JPGHill IPTG.JPG

2-3.Dry : Simulation

2-4.Wet : Assay

Simulationを満たすための他のパラメータが現在のプラスミドで可能であるかを、大腸菌を用いた実験で確認した。

2-5.Future works

3,About our team


Students
Satoru Akama | Kenichiro Iwasaki | Hiroki Kawahara | Hajime Nakatani

Advisors: Last year's member
Syotarou Ayukawa | Akio Kobayashi

Instructors
[http://www.sb.dis.titech.ac.jp/ Daisuke Kiga] | [http://www.es.dis.titech.ac.jp/ Masayuki Yamamura] | [http://nicosia.is.s.u-tokyo.ac.jp/~hagiya/ Masami Hagiya] | [http://www.sb.dis.titech.ac.jp/ Masahiko Uchiyama]

Links sunaba