Tokyo Tech
From 2007.igem.org
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===[[Tokyo/Approach |Approach]]:We alternated approach of Wet and Dry.=== | ===[[Tokyo/Approach |Approach]]:We alternated approach of Wet and Dry.=== | ||
- | ===2-0.Wet :[[Tokyo/Hybrid promoter|Hybrid promoter]]=== | + | ===2-0.Wet : [[Tokyo/Hybrid promoter|Hybrid promoter]]=== |
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Revision as of 04:57, 21 October 2007
1,ProjectConcept: Ant society and Pareto's principleModel : Bacterial society that follows Pareto's principleRequirements: bistability and cell-cell communicationGenetic circuit:Quorum SensingOur construction parts are Here!!! |
2,WorksApproach:We alternated approach of Wet and Dry.2-0.Wet : Hybrid promoter
2-1.Dry :Formulation2-2.WetAssayWe decided the parameter of hill coefficients by the assay of AHL & IPTG2-3.DrySimulation:
wet実験で求められたパラメータを用いて、集団が怠け者の細胞のみとなった場合に、一度不安定化して、続いて働き者と怠け者になれることをStocastic Simulationで確認した。このシステムの挙動に必要な他のパラメータ範囲が判明した。
2-4.WetAssay:
Simulationを満たすための他のパラメータが現在のプラスミドで可能であるかを、大腸菌を用いた実験で確認した。
2-5.Future works3,About our team
Advisors: Last year's member
Instructors
Links sunaba |