Tokyo Tech
From 2007.igem.org
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- | ===[[Tokyo/Approach |Approach]]:We have alternated/combined Wet and Dry experiments to achieve our goal.=== | + | ===[[Tokyo/Approach |Approach]]:<br>We have alternated/combined Wet and Dry experiments to achieve our goal.=== |
===0.<span style="color:#ff33cc;">Wet </span>: [[Tokyo/Hybrid promoter|Hybrid promoter]]=== | ===0.<span style="color:#ff33cc;">Wet </span>: [[Tokyo/Hybrid promoter|Hybrid promoter]]=== |
Revision as of 01:29, 22 October 2007
1,ProjectConcept & ModelWe made bacterial society that follows Pareto's principle, for example, Ant societyRequirementsOur requirements for construction this model are two things, bistability and coexistence by cell-cell communicationGenetic circuitWe constructed the genetic circuit to fill its requirements. Therefore we made new partsOur construction parts are Here!!!Our GOALWe indicated our goal of the project by the simulation!! The simulation proved that our genetic circuit have existence of solution. |
2,Works Approach:
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We need to develop the promoter that can receive two input, AHL & reperssor. We tryed to create and successed!! |
1.Dry : Formulation
We writed the Formulation
2.Wet : Assay
We decided the parameter of hill coefficients by the assay of AHL & IPTG
3.Dry : Simulation
wet実験で求められたパラメータを用いて、集団が怠け者の細胞のみとなった場合に、一度不安定化して、続いて働き者と怠け者になれることをStocastic Simulationで確認した。このシステムの挙動に必要な他のパラメータ範囲が判明した。
By stochastic simulation using parameters obtained from our wet experiments we have confirmed that the whole system is unstable when there are only idlers left, and then, they become either of workers and idlers. Also we have determined other parameters necessary for the desired behavior of this system.
4.Wet : Assay
Simulationを満たすための他のパラメータが現在のプラスミドで可能であるかを、大腸菌を用いた実験で確認した。
1. By the wet experiments using E. coli, we testified whether our current plasmids could satisfy the other required conditions (for the desired behavior of the system) estimated by the simulation.
2. We tested whether our plasmids gave the other parameters to satisfy the simulation by the experiments using E. coli.
5.Future works
3,About our team
Students
Satoru Akama | Kenichiro Iwasaki | Hiroki Kawahara | Hajime Nakatani
Advisors: Last year's member
Syotarou Ayukawa | Akio Kobayashi
Instructors
[http://www.sb.dis.titech.ac.jp/ Daisuke Kiga] | [http://www.es.dis.titech.ac.jp/ Masayuki Yamamura] | [http://nicosia.is.s.u-tokyo.ac.jp/~hagiya/ Masami Hagiya] | [http://www.sb.dis.titech.ac.jp/ Masahiko Uchiyama]
Links sunaba