Week 10
From 2007.igem.org
(Difference between revisions)
Line 85: | Line 85: | ||
*New control digestion for: | *New control digestion for: | ||
- | -[ http://partsregistry.org/Part:BBa_I763019 I763019] with Xba/Spe; | + | -[http://partsregistry.org/Part:BBa_I763019 I763019] with Xba/Spe; |
- | - [http://partsregistry.org/Part:BBa_I763019 I763019] with Xba/Pst1; | + | -[http://partsregistry.org/Part:BBa_I763019 I763019] with Xba/Pst1; |
- | - [http://partsregistry.org/Part:BBa_I763021 I763021] with Eco/Spe; | + | -[http://partsregistry.org/Part:BBa_I763021 I763021] with Eco/Spe; |
- | - [http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Spe; | + | -[http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Spe; |
- | - [http://partsregistry.org/Part:BBa_I763030 I763030] with Eco/Spe; | + | -[http://partsregistry.org/Part:BBa_I763030 I763030] with Eco/Spe; |
*Band extraction for [http://partsregistry.org/Part:BBa_I763019 I763019] (digested with Xba/Spe and with Xba/Pst1), [http://partsregistry.org/Part:BBa_I763026 I763026]; | *Band extraction for [http://partsregistry.org/Part:BBa_I763019 I763019] (digested with Xba/Spe and with Xba/Pst1), [http://partsregistry.org/Part:BBa_I763026 I763026]; | ||
*Ligation O/N for: | *Ligation O/N for: | ||
- | - [http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763019 I763019](http://partsregistry.org/Part:BBa_I763028 I763028]); | + | -[http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763019 I763019](http://partsregistry.org/Part:BBa_I763028 I763028]); |
- | - [http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763004 I763004]([http://partsregistry.org/Part:BBa_I763027 I763027]); | + | -[http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763004 I763004]([http://partsregistry.org/Part:BBa_I763027 I763027]); |
Line 107: | Line 107: | ||
Digestion for: | Digestion for: | ||
- | - [http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Xba; | + | -[http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Xba; |
- | -[ http://partsregistry.org/Part:BBa_I763021 I763021] with Eco/Spe; | + | -[http://partsregistry.org/Part:BBa_I763021 I763021] with Eco/Spe; |
- | -[ http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Spe. | + | -[http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Spe. |
*Band extraction from electrophoresis gel for [http://partsregistry.org/Part:BBa_I763019 I763019] and [http://partsregistry.org/Part:BBa_I763026 I763026]. | *Band extraction from electrophoresis gel for [http://partsregistry.org/Part:BBa_I763019 I763019] and [http://partsregistry.org/Part:BBa_I763026 I763026]. |
Revision as of 20:55, 7 September 2007
- 09/03/07
- Miniprep for:
- Digestion for:
-I763005+I763015 with Spe/Pst1;
-B0015 with Eco/Xba;
- Band extraction;
- Ligation for:
- 09/04/07
- There are colonies for every ligations.
- Meeting.
- 09/05/07
Miniprep for:
- Digestion for:
- I763019 with Eco/Xba;
- I763026 with Eco/Spe;
- I763030 with Eco/Spe;
- I763011 with Xba/Pst1;
- R0051+ I13507(I763007) with Xba/Pst1;
- I763021 with Eco/Spe;
- Control digestion for I763019 with Eco/Pst1;
- Band extraction for
- I763019;
- I763026;
- I763011;
- New control digestion for:
-I763019 with Xba/Spe;
-I763019 with Xba/Pst1;
-I763021 with Eco/Spe;
-I763026 with Eco/Spe;
-I763030 with Eco/Spe;
-I763026+ I763019(http://partsregistry.org/Part:BBa_I763028 I763028]);
- 09/06/07
Digestion for:
-I763019 with Eco/Xba;
-I763021 with Eco/Spe;
-I763026 with Eco/Spe.
- Band extraction from electrophoresis gel for I763019 and I763026.
- Ligation for I763027 with new extracts.
- Transformations for the new ligations.
- 09/07/07