Week 10
From 2007.igem.org
| (14 intermediate revisions not shown) | |||
| Line 21: | Line 21: | ||
-[http://partsregistry.org/Part:BBa_B0015 B0015] with Eco/Xba; | -[http://partsregistry.org/Part:BBa_B0015 B0015] with Eco/Xba; | ||
| - | *Band extraction; | + | *Band extraction from gel for all digestion; |
*Ligation for: | *Ligation for: | ||
| Line 42: | Line 42: | ||
*There are colonies for every ligations. | *There are colonies for every ligations. | ||
*Meeting. | *Meeting. | ||
| + | |||
::'''09/05/07''' | ::'''09/05/07''' | ||
| Line 73: | Line 74: | ||
*Control digestion for [http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Pst1; | *Control digestion for [http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Pst1; | ||
| - | *Band extraction for | + | *Band extraction from gel for: |
- [http://partsregistry.org/Part:BBa_I763019 I763019]; | - [http://partsregistry.org/Part:BBa_I763019 I763019]; | ||
| Line 98: | Line 99: | ||
*Ligation O/N for: | *Ligation O/N for: | ||
| - | -[http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763019 I763019](http://partsregistry.org/Part:BBa_I763028 I763028]); | + | -[http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763019 I763019]([http://partsregistry.org/Part:BBa_I763028 I763028]); |
-[http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763004 I763004]([http://partsregistry.org/Part:BBa_I763027 I763027]); | -[http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763004 I763004]([http://partsregistry.org/Part:BBa_I763027 I763027]); | ||
| Line 116: | Line 117: | ||
*Ligation for [http://partsregistry.org/Part:BBa_I763027 I763027] with new extracts. | *Ligation for [http://partsregistry.org/Part:BBa_I763027 I763027] with new extracts. | ||
*Transformations for the new ligations. | *Transformations for the new ligations. | ||
| + | |||
| + | |||
::'''09/07/07''' | ::'''09/07/07''' | ||
| + | *Glycerol stocks preparation; | ||
| + | *We check without plasmid [[bacteria]] [[Growth Curve - without plasmid bacteria | growth curve]] to individuate a model parameter. | ||
| + | |||
| - | [[Bologna|Back]] | + | [[Bologna#Diary | Back]] |
Latest revision as of 15:53, 26 October 2007
- 09/03/07
- Miniprep for:
-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S0100 S0100];
-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S03520 S03520];
-[http://partsregistry.org/Part:BBa_I763005 I763005]+[http://partsregistry.org/Part:BBa_I763015 I763015];
-[http://partsregistry.org/Part:BBa_J52034 J52034]+[http://partsregistry.org/Part:BBa_E0022 E0022];
- Digestion for:
-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S0100 S0100] with Eco/Spe;
-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S03520 S03520] with Eco/Spe;
-[http://partsregistry.org/Part:BBa_I763005 I763005]+[http://partsregistry.org/Part:BBa_I763015 I763015] with Spe/Pst1;
-[http://partsregistry.org/Part:BBa_J52034 J52034]+[http://partsregistry.org/Part:BBa_E0022 E0022] with Spe/Pst1;
-[http://partsregistry.org/Part:BBa_B0015 B0015] with Eco/Xba;
- Band extraction from gel for all digestion;
- Ligation for:
-[http://partsregistry.org/Part:BBa_I763025 I763025]+[http://partsregistry.org/Part:BBa_B0015 B0015]([http://partsregistry.org/Part:BBa_I763026 I763026]);
-[http://partsregistry.org/Part:BBa_I763024 I763024] +[http://partsregistry.org/Part:BBa_B0015 B0015]([http://partsregistry.org/Part:BBa_I763030 I763030]);
-[http://partsregistry.org/Part:BBa_I763016 I763016]+[http://partsregistry.org/Part:BBa_I763020 I763020]([http://partsregistry.org/Part:BBa_I763019 I763019]);
-[http://partsregistry.org/Part:BBa_I763009 I763009]+[http://partsregistry.org/Part:BBa_P0412 P0412]([http://partsregistry.org/Part:BBa_I763021 I763021]);
-[http://partsregistry.org/Part:BBa_I763009 I763009]+[http://partsregistry.org/Part:BBa_S0100 S0100]([http://partsregistry.org/Part:BBa_I763023 I763023]);
-[http://partsregistry.org/Part:BBa_I763009 I763009]+[http://partsregistry.org/Part:BBa_S03520 S03520]([http://partsregistry.org/Part:BBa_I763022 I763022]);
-[http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_I763020 I763020]([http://partsregistry.org/Part:BBa_I763011 I763011]);
- 09/04/07
- There are colonies for every ligations.
- Meeting.
- 09/05/07
Miniprep for:
- [http://partsregistry.org/Part:BBa_I763016 I763016]+ [http://partsregistry.org/Part:BBa_I763020 I763020] ([http://partsregistry.org/Part:BBa_I763019 I763019]);
- [http://partsregistry.org/Part:BBa_I763025 I763025]+ [http://partsregistry.org/Part:BBa_B0015 B0015] ([http://partsregistry.org/Part:BBa_I763026 I763026]);
- [http://partsregistry.org/Part:BBa_I763024 I763024]+ [http://partsregistry.org/Part:BBa_B0015 B0015] ([http://partsregistry.org/Part:BBa_I763030 I763030]);
- [http://partsregistry.org/Part:BBa_R0051 R0051]+ [http://partsregistry.org/Part:BBa_I763020 I763020] ([http://partsregistry.org/Part:BBa_I763011 I763011]);
- [http://partsregistry.org/Part:BBa_I763009 I763009]+ [http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763021 I763021]);
- [http://partsregistry.org/Part:BBa_I763009 I763009]+ [http://partsregistry.org/Part:BBa_S03520 S03520]([http://partsregistry.org/Part:BBa_I763022 I763022]);
- Digestion for:
- [http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Xba;
- [http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Spe;
- [http://partsregistry.org/Part:BBa_I763030 I763030] with Eco/Spe;
- [http://partsregistry.org/Part:BBa_I763011 I763011] with Xba/Pst1;
- [http://partsregistry.org/Part:BBa_R0051 R0051]+ [http://partsregistry.org/Part:BBa_I13507 I13507]([http://partsregistry.org/Part:BBa_I763007 I763007]) with Xba/Pst1;
- [http://partsregistry.org/Part:BBa_I763021 I763021] with Eco/Spe;
- Control digestion for [http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Pst1;
- Band extraction from gel for:
- [http://partsregistry.org/Part:BBa_I763019 I763019];
- [http://partsregistry.org/Part:BBa_I763026 I763026];
- [http://partsregistry.org/Part:BBa_I763011 I763011];
- [http://partsregistry.org/Part:BBa_R0051 R0051]+ [http://partsregistry.org/Part:BBa_I13507 I13507] ([http://partsregistry.org/Part:BBa_I763007 I763007]);
- New control digestion for:
-[http://partsregistry.org/Part:BBa_I763019 I763019] with Xba/Spe;
-[http://partsregistry.org/Part:BBa_I763019 I763019] with Xba/Pst1;
-[http://partsregistry.org/Part:BBa_I763021 I763021] with Eco/Spe;
-[http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Spe;
-[http://partsregistry.org/Part:BBa_I763030 I763030] with Eco/Spe;
- Band extraction for [http://partsregistry.org/Part:BBa_I763019 I763019] (digested with Xba/Spe and with Xba/Pst1), [http://partsregistry.org/Part:BBa_I763026 I763026];
- Ligation O/N for:
-[http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763019 I763019]([http://partsregistry.org/Part:BBa_I763028 I763028]);
-[http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763004 I763004]([http://partsregistry.org/Part:BBa_I763027 I763027]);
- 09/06/07
Digestion for:
-[http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Xba;
-[http://partsregistry.org/Part:BBa_I763021 I763021] with Eco/Spe;
-[http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Spe.
- Band extraction from electrophoresis gel for [http://partsregistry.org/Part:BBa_I763019 I763019] and [http://partsregistry.org/Part:BBa_I763026 I763026].
- Ligation for [http://partsregistry.org/Part:BBa_I763027 I763027] with new extracts.
- Transformations for the new ligations.
- 09/07/07
- Glycerol stocks preparation;
- We check without plasmid bacteria growth curve to individuate a model parameter.
