Week 16
From 2007.igem.org
(Difference between revisions)
Line 35: | Line 35: | ||
::-[http://partsregistry.org/Part:BBa_I763005 I763005]; | ::-[http://partsregistry.org/Part:BBa_I763005 I763005]; | ||
::-[http://partsregistry.org/Part:BBa_I763031 I763031]. | ::-[http://partsregistry.org/Part:BBa_I763031 I763031]. | ||
- | Gel electrophoresis of the fragments above. | + | *Gel electrophoresis of the fragments above. |
Line 69: | Line 69: | ||
::- [http://partsregistry.org/Part:BBa_I763035 I763035]. | ::- [http://partsregistry.org/Part:BBa_I763035 I763035]. | ||
- | Gel electrophoresis of the fragments above. | + | *Gel electrophoresis of the fragments above. |
Revision as of 14:57, 24 October 2007
- 10/15/07
- O/N ligations for:
- 10/16/07
- Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.
- 10/17/07
- Miniprep of:
- We inoculate:
- Control Digestion of the following Minipreps for results impagination:
- Gel electrophoresis of the fragments above.
- 10/18/07
- Miniprep:
- Digestion for:
- -I763029 (Eco/Pst1)
- Band extraction of:
- -I763029 (Eco/Pst1)
- Ligation for:
- Ligation for:
- Control Digestion of the following Minipreps for results impagination:
- Gel electrophoresis of the fragments above.
- 10/19/07
- Transformation: