Week 8

From 2007.igem.org

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::'''08/20/07'''
::'''08/20/07'''
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*I763010 and C0012 trasformation.  
+
*[http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012] transformation.  
::'''08/21/07'''
::'''08/21/07'''
-
*There isn't colonies for I763010 and C0012. we can't amplify this biobrick;
+
*There are no colonies for [http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012]. We can't amplify these biobricks.
-
*J04631 digestion;
+
*[http://partsregistry.org/Part:BBa_J04631 J04631] digestion.
-
*band extraction;
+
*Band extraction.
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*R0051+P0412 (I763010)ligation.
+
*[http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation.
Line 14: Line 14:
::'''08/22/07'''
::'''08/22/07'''
-
 
+
*There are no colonies for [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010])
::'''08/23/07'''
::'''08/23/07'''
 +
*A new [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation with a new protocol (3h 15°C and 1.2ul T4 Ligase).
 +
*[http://partsregistry.org/Part:BBa_R0051 R0051], [http://partsregistry.org/Part:BBa_I763012 I763012] digestion with Spe/Pst1.
 +
*[http://partsregistry.org/Part:BBa_P0412 P0412], [http://partsregistry.org/Part:BBa_J04631 J04631] digestion with Xba/Pst1.
 +
*Run and extraction from gel.
 +
*Transformation of [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligations.
Line 24: Line 29:
      
      
::'''08/24/07'''
::'''08/24/07'''
 +
*We plan new amplifications.
-
 
+
[[Bologna#Diary | Back]]
-
[[Bologna | Back]]
+

Latest revision as of 15:52, 26 October 2007

08/20/07
  • [http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012] transformation.


08/21/07
  • There are no colonies for [http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012]. We can't amplify these biobricks.
  • [http://partsregistry.org/Part:BBa_J04631 J04631] digestion.
  • Band extraction.
  • [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation.



08/22/07
  • There are no colonies for [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010])


08/23/07
  • A new [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation with a new protocol (3h 15°C and 1.2ul T4 Ligase).
  • [http://partsregistry.org/Part:BBa_R0051 R0051], [http://partsregistry.org/Part:BBa_I763012 I763012] digestion with Spe/Pst1.
  • [http://partsregistry.org/Part:BBa_P0412 P0412], [http://partsregistry.org/Part:BBa_J04631 J04631] digestion with Xba/Pst1.
  • Run and extraction from gel.
  • Transformation of [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligations.



08/24/07
  • We plan new amplifications.


Back