Week 8

From 2007.igem.org

(Difference between revisions)
Line 1: Line 1:
::'''08/20/07'''
::'''08/20/07'''
-
*I763010 and C0012 trasformation.  
+
*[http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012] trasformation.  
::'''08/21/07'''
::'''08/21/07'''
-
*There isn't colonies for I763010 and C0012. we can't amplify this biobrick.
+
*There isn't colonies for [http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012]. we can't amplify this biobrick.
-
*J04631 digestion.  
+
*[http://partsregistry.org/Part:BBa_J04631 J04631] digestion.  
*Band extraction.  
*Band extraction.  
-
*R0051+P0412 (I763010)ligation.
+
*[http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010])ligation.
Line 14: Line 14:
::'''08/22/07'''
::'''08/22/07'''
-
*There isn't any colony for R0051+P0412 (I763010)
+
*There isn't any colony for [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010])
::'''08/23/07'''
::'''08/23/07'''
-
*A new R0051+P0412 (I763010) ligation with a new protocol (3h 15°C and 1.2 ul T4 Ligase).
+
*A new [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation with a new protocol (3h 15°C and 1.2 ul T4 Ligase).
-
*R0051, I763012 digestion with Spe/Pst1.
+
*[http://partsregistry.org/Part:BBa_R0051 R0051], [http://partsregistry.org/Part:BBa_I763012 I763012] digestion with Spe/Pst1.
-
*P0412, J04631 digestion with Xba/Pst1.
+
*[http://partsregistry.org/Part:BBa_P0412 P0412], [http://partsregistry.org/Part:BBa_J04631 J04631] digestion with Xba/Pst1.
*Run and extraction from gel.
*Run and extraction from gel.
-
*Transformation of R0051+P0412 (I763010) ligations.
+
*Transformation of [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligations.

Revision as of 11:56, 28 August 2007

08/20/07
  • [http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012] trasformation.


08/21/07
  • There isn't colonies for [http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012]. we can't amplify this biobrick.
  • [http://partsregistry.org/Part:BBa_J04631 J04631] digestion.
  • Band extraction.
  • [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010])ligation.



08/22/07
  • There isn't any colony for [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010])


08/23/07
  • A new [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation with a new protocol (3h 15°C and 1.2 ul T4 Ligase).
  • [http://partsregistry.org/Part:BBa_R0051 R0051], [http://partsregistry.org/Part:BBa_I763012 I763012] digestion with Spe/Pst1.
  • [http://partsregistry.org/Part:BBa_P0412 P0412], [http://partsregistry.org/Part:BBa_J04631 J04631] digestion with Xba/Pst1.
  • Run and extraction from gel.
  • Transformation of [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligations.



08/24/07
  • We plan new amplifications.


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