Chiba

From 2007.igem.org

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*BBa_T9002(Normal Receiver)
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*Inverter aiiA Receiver
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Revision as of 08:07, 23 October 2007

Chiba logo.png

Introduction | Project Design | Engeneering Flagella | Quorum Sensing | Our Goal || Team Members | メンバ連絡簿

Concept

Marimo in the lake

Chiba University iGEM07 team's project is to make a Marimo-ish gathering of bacteria. Marimo is a green spherical shaped algae, which is a popular living organism in Japan as a National Treasure. When you come to Japan, you absolutely come across to Marimo in Aquarisms, TV programs or Mangas :)


How Marimos are Made

球形の毬藻は,他の崩れた毬藻断片を核として放射状に伸びることによって出来る.よって成長後の球形毬藻は全て一様で核が存在しない.ちなみに最初の無核球形毬藻は,他の泥や砂などを核として成長し崩れた毬藻断片を核としたと考えられている.(イメージ図)

more about marimos...

References:

  • Yoshio Sakai, Marimo no Kagaku, 北海道大学図書刊行会, 1991


Why Make a "Marimo Bacteria"?

大腸菌は固体上やプレート上でそれぞれバイオフィルムやコロニーを形成するなど,固体上で集合体を生じることは知られている.しかし液体中で細胞自身が集合する例は調べる限りでは見当たらない.我々は大腸菌のみで毬藻のような独立した有限の形の集合体をつくることを目指した. (イメージ図)


Project

What our system requires

1.Adhesives of Bacteria: make a His-tagged Flagella

Fig1. His-tagged flagella

>>go to the Flagella Engeneering page

2.Size Controller: Generate AHL Concentration in Quorum Sensing (絵)

Genetic Circuit

Chiba marimosystem.png

Sender

Send gene circuit.jpg

Reciever

  • BBa_T9002(Normal Receiver)

BBa T9002.jpg

  • Sensitive Receiver

Mutant rec.jpg

  • Inverter aiiA Receiver

Rec inv aiia.jpg