Chiba/Weekly
From 2007.igem.org
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== Week1(8/16~8/23) == | == Week1(8/16~8/23) == | ||
- | + | '''Monday,August,17,2007''' | |
*pUC-FliCのPCR | *pUC-FliCのPCR | ||
{| border="1" cellspacing="0" cellpadding="4" align="center" | {| border="1" cellspacing="0" cellpadding="4" align="center" | ||
|- | |- | ||
!F/R | !F/R | ||
- | !207 | + | !FliC-207 |
- | !228 | + | !FliC-228 |
- | !240 | + | !FliC-240 |
- | !252 | + | !FliC-252 |
- | !267 | + | !FliC-267 |
|- | |- | ||
- | |207 | + | |FliC-207 |
|① | |① | ||
| | | | ||
Line 18: | Line 18: | ||
| | | | ||
|- | |- | ||
- | |228 | + | |FliC-228 |
- | + | ||
|② | |② | ||
+ | |③ | ||
| | | | ||
| | | | ||
| | | | ||
|- | |- | ||
- | |240 | + | |FliC-240 |
- | | | + | |④ |
- | | | + | |⑤ |
- | | | + | |⑥ |
| | | | ||
| | | | ||
|- | |- | ||
- | |252 | + | |FliC-252 |
- | | | + | |⑦ |
- | | | + | |⑧ |
- | | | + | |⑨ |
- | | | + | |⑩ |
| | | | ||
|- | |- | ||
- | |267 | + | |FliC-267 |
- | | | + | |⑪ |
- | | | + | |⑫ |
- | | | + | |⑬ |
- | | | + | |⑭ |
- | | | + | |⑮ |
+ | |- | ||
|} | |} | ||
+ | |||
+ | {| border="1" cellspacing="0" cellpadding="4" align="center" | ||
+ | |- | ||
+ | !Recipe(単位:μ) | ||
+ | !① | ||
+ | !② | ||
+ | !③ | ||
+ | !④ | ||
+ | !⑤ | ||
+ | !⑥ | ||
+ | !⑦ | ||
+ | !⑧ | ||
+ | !⑨ | ||
+ | !⑩ | ||
+ | !⑪ | ||
+ | !⑫ | ||
+ | !⑬ | ||
+ | !⑭ | ||
+ | !⑮ | ||
+ | |- | ||
+ | |pUC19-FliC | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |- | ||
+ | |FliC-207F | ||
+ | |5 | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | |- | ||
+ | |FliC-207R | ||
+ | |5 | ||
+ | |5 | ||
+ | | | ||
+ | |5 | ||
+ | | | ||
+ | | | ||
+ | |5 | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | |5 | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | |- | ||
+ | |FliC-228F | ||
+ | | | ||
+ | |5 | ||
+ | |5 | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | |- | ||
+ | |FliC-228R | ||
+ | | | ||
+ | | | ||
+ | |5 | ||
+ | | | ||
+ | |5 | ||
+ | | | ||
+ | | | ||
+ | |5 | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | |5 | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | |- | ||
+ | |FliC-240F | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | |5 | ||
+ | |5 | ||
+ | |5 | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | |- | ||
+ | |FliC-240R | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | |5 | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | |5 | ||
+ | | | ||
+ | | | ||
+ | |5 | ||
+ | | | ||
+ | | | ||
+ | |- | ||
+ | |FliC-252F | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | |5 | ||
+ | |5 | ||
+ | |5 | ||
+ | |5 | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | |- | ||
+ | |FliC-252R | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | |5 | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | |5 | ||
+ | | | ||
+ | |- | ||
+ | |FliC-267F | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | |5 | ||
+ | |5 | ||
+ | |5 | ||
+ | |5 | ||
+ | |5 | ||
+ | |- | ||
+ | |FliC-267R | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | |5 | ||
+ | |- | ||
+ | |10×Buffer | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |- | ||
+ | |dNTPmix | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |10 | ||
+ | |- | ||
+ | |Vent | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |1 | ||
+ | |- | ||
+ | |dH2O | ||
+ | |68 | ||
+ | |68 | ||
+ | |68 | ||
+ | |68 | ||
+ | |68 | ||
+ | |68 | ||
+ | |68 | ||
+ | |68 | ||
+ | |68 | ||
+ | |68 | ||
+ | |68 | ||
+ | |68 | ||
+ | |68 | ||
+ | |68 | ||
+ | |68 | ||
+ | |- | ||
+ | |Total | ||
+ | |100 | ||
+ | |100 | ||
+ | |100 | ||
+ | |100 | ||
+ | |100 | ||
+ | |100 | ||
+ | |100 | ||
+ | |100 | ||
+ | |100 | ||
+ | |100 | ||
+ | |100 | ||
+ | |100 | ||
+ | |100 | ||
+ | |100 | ||
+ | |100 | ||
+ | |- | ||
+ | |} | ||
== Week2(8/24~8/31) == | == Week2(8/24~8/31) == | ||
Line 56: | Line 350: | ||
== Week4(9/9~9/15) == | == Week4(9/9~9/15) == | ||
+ | *Swaming test | ||
+ | #FliC-267His-RBSプラスミドをJW1908(KEIO⊿FliC)にTransformation(Positive Control=ASKA,Negative Control=KEIO-JW1908にFliC-267HisをTransformしたもの) | ||
+ | #LB培地で37℃12h2ml培養 | ||
+ | #トリプトン培地(Agar0.25%)の中央に1μinoculate | ||
+ | #30℃、24-48h,Taxis | ||
+ | #Taxis ling を観察 | ||
+ | *'''Sample''' | ||
+ | [[Image:swam4-2-2.jpg]] | ||
== Week5(9/16~9/22) == | == Week5(9/16~9/22) == |
Latest revision as of 02:48, 12 September 2007
Contents |
Week1(8/16~8/23)
Monday,August,17,2007
- pUC-FliCのPCR
F/R | FliC-207 | FliC-228 | FliC-240 | FliC-252 | FliC-267 |
---|---|---|---|---|---|
FliC-207 | ① | ||||
FliC-228 | ② | ③ | |||
FliC-240 | ④ | ⑤ | ⑥ | ||
FliC-252 | ⑦ | ⑧ | ⑨ | ⑩ | |
FliC-267 | ⑪ | ⑫ | ⑬ | ⑭ | ⑮ |
Recipe(単位:μ) | ① | ② | ③ | ④ | ⑤ | ⑥ | ⑦ | ⑧ | ⑨ | ⑩ | ⑪ | ⑫ | ⑬ | ⑭ | ⑮ |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
pUC19-FliC | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
FliC-207F | 5 | ||||||||||||||
FliC-207R | 5 | 5 | 5 | 5 | 5 | ||||||||||
FliC-228F | 5 | 5 | |||||||||||||
FliC-228R | 5 | 5 | 5 | 5 | |||||||||||
FliC-240F | 5 | 5 | 5 | ||||||||||||
FliC-240R | 5 | 5 | 5 | ||||||||||||
FliC-252F | 5 | 5 | 5 | 5 | |||||||||||
FliC-252R | 5 | 5 | |||||||||||||
FliC-267F | 5 | 5 | 5 | 5 | 5 | ||||||||||
FliC-267R | 5 | ||||||||||||||
10×Buffer | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
dNTPmix | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
Vent | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
dH2O | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 |
Total | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
Week2(8/24~8/31)
Week3(9/1~9/8)
Week4(9/9~9/15)
- Swaming test
- FliC-267His-RBSプラスミドをJW1908(KEIO⊿FliC)にTransformation(Positive Control=ASKA,Negative Control=KEIO-JW1908にFliC-267HisをTransformしたもの)
- LB培地で37℃12h2ml培養
- トリプトン培地(Agar0.25%)の中央に1μinoculate
- 30℃、24-48h,Taxis
- Taxis ling を観察
- Sample